17
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Accessing genetic information with high-density DNA arrays.

      Science (New York, N.Y.)

      Algorithms, Base Composition, Base Sequence, Cloning, Molecular, DNA, Mitochondrial, genetics, Fluorescein, Fluoresceins, Gene Expression, Genetic Variation, Genome, Humans, Mitochondria, Nucleic Acid Hybridization, Oligonucleotide Probes, Phycoerythrin, Polymerase Chain Reaction, Polymorphism, Genetic, Sequence Analysis, DNA

      Read this article at

      ScienceOpenPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Rapid access to genetic information is central to the revolution taking place in molecular genetics. The simultaneous analysis of the entire human mitochondrial genome is described here. DNA arrays containing up to 135,000 probes complementary to the 16.6-kilobase human mitochondrial genome were generated by light-directed chemical synthesis. A two-color labeling scheme was developed that allows simultaneous comparison of a polymorphic target to a reference DNA or RNA. Complete hybridization patterns were revealed in a matter of minutes. Sequence polymorphisms were detected with single-base resolution and unprecedented efficiency. The methods described are generic and can be used to address a variety of questions in molecular genetics including gene expression, genetic linkage, and genetic variability.

          Related collections

          Author and article information

          Journal
          8849452

          Comments

          Comment on this article