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      Detection of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil

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          Abstract

          To determine the presence of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil, 80 animals slaughtered in the public slaughterhouse of Patos city were used. Before slaughter, blood samples were collected by jugular venopuncture from each animal, and after slaughter, testicles, epidydimus and uterus were aseptically collected. For the serological diagnosis of B. ovis and B. abortus infections, the agar gel immunodiffusion (AGID) and Rose Bengal (RBT) tests were carried out, respectively. In addition, microbiological culture and polymerase chain reaction (PCR) were performed on testicle, epidydimus and uterus samples. Six animals (7.5%) tested positive for the presence of B. ovis antibodies and all animals tested negative for the presence of B. abortus antibodies. One AGID-positive animal tested positive at uterine swab culture. PCR was able to amplify DNA of Brucella spp. from the pool of testicle, epidydimus and uterus samples from AGID-positive animals. This is the first report of isolation and detection of B. ovis DNA by PCR in ovine from the Northeast region of Brazil.

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          Brucellosis in Brazil.

          This paper reviews the epidemiology of bovine, swine, ovine, caprine, and canine brucellosis in Brazil. The zoonotic aspects of Brucella infection in Brazil is also discussed. Emphasis is given to the new program for the control of brucellosis in cattle and buffaloes that is likely to provide important insights into the prospects and strategies for controlling brucellosis in developing countries. Copyright 2002 Elsevier Science B.V.
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            Instituto Brasileiro de Geografia e Estatística (IBGE)

            (2015)
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              Diagnosis of canine brucellosis: comparison between serological and microbiological tests and a PCR based on primers to 16S-23S rDNA interspacer.

              A pair of primers directed to 16S-23S rDNA interspacer (ITS) was designed directed to Brucella genetic sequences in order to develop a polymerase chain reaction (PCR) putatively capable of amplifying DNA from any Brucella species. Nucleic acid extracts from whole-blood from naive dogs were spiked with decreasing amounts of Brucella canis RM6/66 DNA and the resulting solutions were tested by PCR. In addition, the ability of PCR to amplify Brucella spp. genetic sequences from naturally infected dogs was evaluated using 210 whole-blood samples of dogs from 19 kennels. The whole-blood samples collected were subjected to blood culture and PCR. Serodiagnosis was performed using the rapid slide agglutination test with and without 2-mercaptoethanol. The DNA from whole blood was extracted using proteinase-K, sodium dodecyl sulphate and cetyl trimethyl ammonium bromide followed by phenol-chloroform purification. The PCR was capable of detecting as little as 3.8 fg of Brucella DNA mixed with 450 ng of host DNA. Theoretically, 3.8 fg of Brucella DNA represents the total genomic mass of fewer than two bacterial cells. The PCR diagnostic sensitivity and specificity were 100%. From the results observed in the present study, we conclude that PCR could be used as confirmatory test for diagnosis of B. canis infection.
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                Author and article information

                Journal
                Braz J Microbiol
                Braz. J. Microbiol
                bjm
                bjm
                Brazilian Journal of Microbiology
                Sociedade Brasileira de Microbiologia
                1517-8382
                1678-4405
                Apr-Jun 2010
                1 June 2010
                : 41
                : 2
                : 365-367
                Affiliations
                [1 ]Unidade Acadêmica de Medicina Veterinária, Centro de Saúde e Tecnologia Rural, Universidade Federal de Campina Grande , Patos, PB, Brasil
                [2 ]Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia , Universidade de São Paulo, SP, Brasil
                Author notes
                * Corresponding Author. Mailing address: Unidade Acadêmica de Medicina Veterinária, Centro de Saúde e Tecnologia Rural, Universidade Federal de Campina Grande, Patos, Paraíba, Brazil.; E-mail: clebertja@ 123456uol.com.br
                Article
                S1517-838220100002000016
                10.1590/S1517-838220100002000016
                3768702
                24031505
                0dcb77e1-f865-4b4b-955b-68bbeb7733d3
                © Sociedade Brasileira de Microbiologia

                All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License

                History
                : 29 September 2008
                : 31 October 2008
                : 07 November 2009
                Categories
                Veterinary Microbiology
                Short Communication

                brucella ovis,isolation,pcr,ovine,paraíba state
                brucella ovis, isolation, pcr, ovine, paraíba state

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