Seroprevalence of Triatoma virus (Dicistroviridae: Cripaviridae) antibodies in Chagas disease patients

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Abstract

Background

Chagas disease is caused by Trypanosoma cruzi, and humans acquire the parasite by exposure to contaminated feces from hematophagous insect vectors known as triatomines. Triatoma virus (TrV) is the sole viral pathogen of triatomines, and is transmitted among insects through the fecal-oral route and, as it happens with T. cruzi, the infected insects release the virus when defecating during or after blood uptake.

Methods

In this work, we analysed the occurrence of anti-TrV antibodies in human sera from Chagas disease endemic and non-endemic countries, and developed a mathematical model to estimate the transmission probability of TrV from insects to man, which ranged between 0.00053 and 0.0015.

Results

Our results confirm that people with Chagas disease living in Bolivia, Argentina and Mexico have been exposed to TrV, and that TrV is unable to replicate in human hosts.

Conclusions

We presented the first experimental evidence of antibodies against TrV structural proteins in human sera.

Electronic supplementary material

The online version of this article (doi:10.1186/s13071-015-0632-9) contains supplementary material, which is available to authorized users.

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Most cited references 22

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Chagas disease in rural areas of Chaco Province, Argentina: epidemiologic survey in humans, reservoirs, and vectors.

Miguel Basombrío, Estefanía R. Zacca, Jorge Marco … (2004)

We studied the seroprevalence of antibodies against Trypanosoma cruzi in the human population along with domiciliary infestation by triatomine bugs in an area endemic for Chagas disease in the Chaco Province of Argentina. In addition, we carried out parasitologic surveys in patients, dogs, wild mammals, and vectors. The mean seroprevalence in humans was 27.81% (109 of 392) and 24.14% (63 of 261) in 1-15-year-old children. The minimum domiciliary infestation rate was 13.33%, with certain areas reaching 53.85%. The prevalence was 15.09% (16 of 106) in dogs and 35.71% (10 of 28) in opossums. Infection with T. cruzi was detected in 30.10% (59 of 196) of the Triatoma infestans tested. Compared with nationwide studies, our data suggest that 1) there are zones requiring immediate sanitary action, and 2) nationwide estimates are based on very heterogeneous epidemiologic situations. This heterogeneity emphasizes the importance of in-depth studies of restricted areas to provide additional information for a better understanding of the present status of Chagas disease in Argentina.

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Capsid protein identification and analysis of mature Triatoma virus (TrV) virions and naturally occurring empty particles.

Emmanuelle Neumann, Kerman Aloria, Ruben Sanchez … (2011)

Triatoma virus (TrV) is a non-enveloped +ssRNA virus belonging to the insect virus family Dicistroviridae. Mass spectrometry (MS) and gel electrophoresis were used to detect the previously elusive capsid protein VP4. Its cleavage sites were established by sequencing the N-terminus of the protein precursor and MS, and its stoichiometry with respect to the other major capsid proteins (VP1-3) was found to be 1:1. We also characterized the polypeptides comprising the naturally occurring non-infectious empty capsids, i.e., RNA-free TrV particles. The empty particles were composed of VP0-VP3 plus at least seven additional polypeptides, which were identified as products of the capsid precursor polyprotein. We conclude that VP4 protein appears as a product of RNA encapsidation, and that defective processing of capsid proteins precludes genome encapsidation. Copyright © 2010 Elsevier Inc. All rights reserved.

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Triatoma virus pathogenicity in laboratory colonies of Triatoma infestans (Hemiptera:Reduviidae).

Armando J Torre, O Muscio, Edward M. Bonder … (1997)

In a survey of wild populations of Triatoma infestans (Klug) in Argentina, 10% were infected with Triatoma virus (TrV). The virus also was detected in a laboratory colony 18 mo after being established, with infection rates up to 100%. Mortality rate was 97.6% in nymphs and the molting process was inhibited, thereby increasing development time. Because the virus was detected in colony nymphs. TrV may be transmitted vertically. However, the higher infection rate in the colony compared with natural populations also indicates other route(s) of transmission.

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Author and article information

Contributors

Jailson FB Querido: jailson.brito.querido@gmail.com

María G Echeverría: mariagabrielaecheverria@yahoo.com.ar

Gerardo A Marti: gerardomarti22@yahoo.com.ar

Rita Medina Costa: ritamedina_costa@hotmail.com

María L Susevich: mlsusevich@hotmail.com

Jorge E Rabinovich: jorge.rabinovich@gmail.com

Aydee Copa: acopa@hotmail.com

Nair A Montaño: namontano@gmail.com

Lineth Garcia: lineth.garcia@gmail.com

Marisol Cordova: mcordova@hotmail.com

Faustino Torrico: foxtorrico@yahoo.com

Rubén Sánchez-Eugenia: r.sanchez.eugenia@gmail.coml

Lissete Sánchez-Magraner: lissetes@hotmail.com

Xabier Muñiz-Trabudua: xabiermt@gmail.com

Ibai López-Marijuan: ibai_87@hotmail.com

Gabriela S Rozas-Dennis: grozas@criba.edu.ar

Patricio Diosque: patricio.diosque@gmail.com

Ana M de Castro: amaria.ana@gmail.com

Carlos Robello: robello@pasteur.edu.uy

Julio S Rodríguez: juliocesar.neurotox@gmail.com

Jaime Altcheh: altcheh@gmail.com

Paz M Salazar-Schettino: pazmar@unam.mx

Marta I Bucio: mbucio@hotmail.com

Bertha Espinoza: besgu@biomedicas.unam.mx

Diego MA Guérin: diego.guerin@ehu.es

Marcelo Sousa Silva: mssilva@ihmt.unl.pt

Journal

Journal ID (nlm-ta): Parasit Vectors

Journal ID (iso-abbrev): Parasit Vectors

Title: Parasites & Vectors

Publisher: BioMed Central (London )

ISSN (Electronic): 1756-3305

Publication date (Electronic): 17 January 2015

Publication date PMC-release: 17 January 2015

Publication date Collection: 2015

Volume: 8

Electronic Location Identifier: 29

Affiliations

[ ]Centre for Malaria and Tropical Diseases, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisbon, Portugal

[ ]Cátedra de Virología, Facultad de Ciencias Veterinarias Universidad Nacional de La Plata (UNLP-CONICET), La Plata, Argentina

[ ]Centro de Estudios Parasitológicos y de Vectores (CEPAVE-CCT- La Plata -CONICET - UNLP), La Plata, Argentina

[ ]Laboratorio de Biología Molecular IIBISMED, Facultad de Medicina, Universidad Mayor de San Simón, Cochabamba, Bolivia

[ ]Facultad de Medicina, Universidad Mayor de San Simón, Cochabamba, Bolivia

[ ]Unidad de Biofísica (UBF, CSIC, UPV-EHU), Leioa, Bizkaia Spain

[ ]Departamento de Biología, Bioquímica y Farmacia, and Grupo Biofísica, Departamento de Física, Universidad Nacional del Sur, Bahía Blanca, Argentina

[ ]Unidad de Epidemiología Molecular del Instituto de Patología Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta, Salta, Argentina

[ ]Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiania, Brazil

[ ]Unidad de Biología Molecular Instituto Pasteur de Montevideo, Mataojo 2020, CP11400 Montevideo, Uruguay

[ ]CENPALAB, Delegación CIMTA, Santiago de Cuba, Cuba

[ ]Parasitologia-Chagas, Hospital de Niños R. Gutierrez, Buenos Aires, Argentina

[ ]Departamento de Microbiología y Parasitología, Facultad de Medicina (LBP-DMP-FM), Universidad Nacional Autónoma de México, Mexico City, DF México

[ ]Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Autónoma de México, Mexico City, DF México

[ ]Departamento de Bioquímica y Biología Molecular, Universidad del País Vasco (EHU/UPV), Bizkaia, Spain

[ ]Programa de Pós-graduação em Bioquímica, Departamento Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, Brazil

Article

Publisher ID: 632

DOI: 10.1186/s13071-015-0632-9

PMC ID: 4351825

PubMed ID: 25595198

SO-VID: 0de05b06-593f-462d-b5f6-e8ebef73173c

License:

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

History

Date received : 29 August 2014

Date accepted : 31 December 2014

Custom metadata

ScienceOpen disciplines: Parasitology

Keywords: triatoma virus,dicistroviridae,trypanosoma cruzi,triatomines,chagas disease,mathematical model,biological control,passive viral exposure

Data availability:

ScienceOpen disciplines: Parasitology

Keywords: triatoma virus, dicistroviridae, trypanosoma cruzi, triatomines, chagas disease, mathematical model, biological control, passive viral exposure

Seroprevalence of Triatoma virus ( Dicistroviridae: Cripaviridae) antibodies in Chagas disease patients

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Abstract

Background

Methods

Results

Conclusions

Electronic supplementary material

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Entomologia Generalis

Most cited references 22

Chagas disease in rural areas of Chaco Province, Argentina: epidemiologic survey in humans, reservoirs, and vectors.

Capsid protein identification and analysis of mature Triatoma virus (TrV) virions and naturally occurring empty particles.

Triatoma virus pathogenicity in laboratory colonies of Triatoma infestans (Hemiptera:Reduviidae).

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