Evaluation of performances of VERSANT HCV RNA 1.0 assay (kPCR) and Roche COBAS AmpliPrep/COBAS TaqMan HCV test v2.0 at low level viremia.

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Abstract

We assess the concordance between low level HCV values obtained using the VERSANT HCV RNA 1.0 Assay (kPCR) and COBAS AmpliPrep/COBAS TaqMan HCV Quantitative Test v2.0. The correlation between the values obtained by the two RT-PCR assays for samples with quantifiable HCV RNA levels revealed that viral load measured by kPCR significantly correlated with that of the CAP/CTM (R=0.644, P<0.0001). The results show a good concordance (n=126/144, 87%); discordant results were mainly observed in the assessment of values below the lower limit of detection of the assays. These variations may have an impact on clinical decisions for patients on HCV triple therapy or interferon- free regimens. It is therefore recommended to monitor individual patients with the same test throughout treatment.

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Journal

Journal ID (iso-abbrev): New Microbiol.

Title: The new microbiologica

ISSN: 1121-7138

ISSN (Print): 1121-7138

Publication date (Electronic): Jul 2016

Volume: 39

Issue: 3

Affiliations

[1 ] Department of Molecular Medicine, Sapienza University of Rome, Italy.

[2 ] Department of Public Health and Infectious Diseases, Sapienza University of Rome, Italy.

[3 ] University Campus Bio-Medico of Rome, Italy.

Article

Publisher Item ID: 496M866

PubMed ID: 27284986

SO-VID: 0e18445a-097a-4e89-8212-fb9e7b637f36

History

Keywords: Real-time PCR,Viral load,VERSANT HCV RNA,HCV,COBAS TaqMan HCV

Data availability:

Keywords: Real-time PCR, Viral load, VERSANT HCV RNA, HCV, COBAS TaqMan HCV

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