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      Accumulation of biomass and four triterpenoids in two-stage cultured Poria cocos mycelia and diuretic activity in rats

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          Abstract

          Poria cocos (Schw.) Wolf, an important medicinal and food fungus, is well known in East Asia. Due to growing market demand, long cultivation period, and consumption of pine trunk during cultivation, developing alternative methods for producing P. cocos and/or its active components is of interest. In the present study, the effects of different culture methods on biomass and accumulation of four triterpenoids were investigated. The ethanol extract of fermented mycelium (EFM) was orally administered to rats. Urine output and concentrations of electrolytes (Na +, K +, and Cl ) were measured. Our results showed that mycelia grew better under continuous shaking culture condition (7.5 g DW·L –1), and higher triterpenoid levels were accumulated in two-stage culture (112 mg·L –1, 2.03%). The optimal starting time of static culture for triterpenoid yield was 4 th d after shaking culture. Single administration of middle and high dose of EFM significantly increased urine output, Na + and Cl excretion, and Na +/K + ratio. These results suggested that ethanol extract of cultured mycelia showed significant diuretic activity in rats and two-stage culture of P. cocos could be an alternative way to produce mycelia and triterpenoids.

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          Author and article information

          Journal
          CJNM
          Chinese Journal of Natural Medicines
          Elsevier
          1875-5364
          20 April 2017
          : 15
          : 4
          : 265-270
          Affiliations
          1School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University; Shenyang 110016, China
          2Pharmaceutical Research Center, Jiangsu Kanion Pharmaceutical Co., Ltd.; Lianyungang 222001, China
          3The Sixth Traditional Chinese Medicines Factory, Zhongxin Pharmaceutical Group Ltd.; Tianjin 300401, China
          Author notes
          *Corresponding author: JIA Jing-Ming, Tel: 86-24-23986501, E-mail: jmingjia@ 123456sina.com

          These authors have no conflict of interest to declare.

          Article
          S1875-5364(17)30043-2
          10.1016/S1875-5364(17)30043-2
          28527511
          Copyright © 2017 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.
          Funding
          Funded by: 54th China Postdoctoral Science Foundation
          Award ID: 2013M541255
          Funded by: Shenyang Pharmaceutical University
          Award ID: ZQN2015034
          This work was financially supported by the post-doctoral program of 54th China Postdoctoral Science Foundation (No. 2013M541255), Career Development Support Program for Young and Middle-aged Teachers of Shenyang Pharmaceutical University (No. ZQN2015034), and the funds from Shenyang Pharmaceutical University and Kanion Pharmaceutical Co. Ltd..
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