In this study, we have analyzed numerous effects of the acupuncture and pressing of traditional Chinese medicine on recovery of delayed muscle soreness in athletes. To implement this idea, a total of 84 athletes with delayed muscle soreness treated in our hospital from January 2018 to June 2020 were included. According to the random number table method, they were divided into the experimental group and the control group: among them, 42 cases were in the experimental group and were treated by TCM acupuncture. In the control group, 42 athletes used rehabilitation training. Compared with serum CK levels in the control group, serum CK levels in the experimental group decreased after training, and there was a significant difference (P < 0.05). After intensive training in the control group (P < 0.05), it indicated that acupuncture and pressing of traditional Chinese medicine was effective in the recovery of fatigue after exercise, which was significantly better than that in the control group. The serum testosterone value of the experimental group before intensive training had no significant difference, but the serum testosterone value of the moxibustion group of male athletes after intensive training was significantly higher than that of the control group (P < 0.05). Female athletes have a low blood testosterone base and are less affected by the gonad axis. There was no statistically significant difference in RPE scores before treatment. After treatment, the RPE score of the experimental group decreased rapidly (P < 0.05), which was statistically significant compared with the control group (P < 0.05). There was no significant difference in VAS scores before treatment. After treatment, the VAS score in the experimental group decreased rapidly (P < 0.05) compared with the control group (P < 0.05). After treatment, compared with the control group, the experimental group had a decrease in calf circumference, an increase in muscle strength, and an increase in the range of motion (P < 0.05). Acupuncture and pressing of traditional Chinese medicine have obvious clinical application value in the treatment of delayed muscle soreness in athletes, and they have great clinical application value. Muscle injury is a common disease of athletes.