Background research may be time consuming but is an essential component in producing a compliant, efficient, and quality biorepository. This article addresses 5 fundamental considerations associated with biorepository development. The process begins with community and stakeholder buy-in. Once this phase is complete and stakeholder approval is achieved, several crucial elements remain. When proceeding, it is important to consider researcher needs, initial facility setup requirements, start-up funding sources, biorepository site visits, regulations, and best practices. Multiple sources should be utilized when exploring these topics and should include guidance documents, regulations, existing literature, and "borrowed knowledge" from others who have been through the process. Thorough research during this initial phase and the subsequent tailoring of this information to fit the specific institution's needs will contribute significantly to the success of the biorepository.