Development of a Bead-Based Multiplex Assay for the Analysis of the Serological Response against the Six Pathogens HAV, HBV, HCV, CMV,  T. gondii , and  H. pylori

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

The spread of infectious diseases and vaccination history are common subjects of epidemiological and immunological research studies. Multiplexed serological assays are useful tools for assessing both current and previous infections as well as vaccination efficacy. We developed a serological multi-pathogen assay for hepatitis A, B and C virus, cytomegalovirus (CMV), Toxoplasma gondii, and Helicobacter pylori using a bead-based multiplex assay format. The multi-pathogen assay consisting of 15 antigens was utilized for the analysis of the serological response in elderly individuals of an influenza vaccination study ( n = 34). The technical assay validation revealed a mean intra-assay precision of coefficient of variation (CV) = 3.2 ± 1.5% and a mean inter-assay precision of CV = 8.2 ± 5.3% across all 15 antigens and all tested samples, indicating a robust test system. Furthermore, the assay shows high sensitivities (ranging between 94% and 100%) and specificities (ranging between 93% and 100%) for the different pathogens. The highest seroprevalence rates in our cohort were observed for hepatitis A virus (HAV; 73.5%), followed by CMV (70.6%), T. gondii (67.6%) and H. pylori (32.4%). Seroprevalences for hepatitis B virus (HBV, 8.8%) and hepatitis C virus (HCV, 0%) were low. The seroprevalences observed in our study were similar to those from other population-based studies in Germany. In summary, we conclude that our multiplex serological assay represents a suitable tool for epidemiological studies.

Related collections

Most cited references 22

Record: found
Abstract: found
Article: not found

Helicobacter pylori.

B E Dunn, H. Cohen, M Blaser (1997)

Helicobacter pylori is a gram-negative bacterium which causes chronic gastritis and plays important roles in peptic ulcer disease, gastric carcinoma, and gastric lymphoma. H. pylori has been found in the stomachs of humans in all parts of the world. In developing countries, 70 to 90% of the population carries H. pylori. In developed countries, the prevalence of infection is lower. There appears to be no substantial reservoir of H. pylori aside from the human stomach. Transmission can occur by iatrogenic, fecal-oral, and oral-oral routes. H. pylori is able to colonize and persist in a unique biological niche within the gastric lumen. All fresh isolates of H. pylori express significant urease activity, which appears essential to the survival and pathogenesis of the bacterium. A variety of tests to diagnose H. pylori infection are now available. Histological examination of gastric tissue, culture, rapid urease testing, DNA probes, and PCR analysis, when used to test gastric tissue, all require endoscopy. In contrast, breath tests, serology, gastric juice PCR, and urinary excretion of [15N]ammonia are noninvasive tests that do not require endoscopy. In this review, we highlight advances in the detection of the presence of the organism and methods of differentiating among types of H. pylori, and we provide a background for appropriate chemotherapy of the infection.

0 comments Cited 155 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

[Epidemiology of hepatitis A, B, and C among adults in Germany: results of the German Health Interview and Examination Survey for Adults (DEGS1)].

R. Paul Ross, R. Zimmermann, O Hamouda … (2013)

Ten years after seroepidemiological data were obtained in the German National Health Interview and Examination Survey 1998 (GNHIES98), German Health Interview and Examination Survey (DEGS1) data contribute to a population-based, representative surveillance of hepatitis A and B immunity and of the serological markers for hepatitis C in Germany. The prevalence of antibodies against the hepatitis A virus is 48.6 %. In comparison to the situation 10 years ago, seroprevalence is significantly higher among 18- to 39-year-old adults and is significantly lower in those aged 50-79 years. The association between age and seroprevalence has changed, indicating a decrease in naturally acquired hepatitis A immunity. Individual and population immunity has to be achieved through vaccination. Prevalence of hepatitis B antibodies indicates that 5.1 % of adults have been exposed to the virus, significantly fewer than 10 years ago (7.9 %). Prevalence of hepatitis B surface antibodies indicates that 22.9 % of adults have been vaccinated against hepatitis B. Vaccination coverage has increased in all age groups and is highest in the younger age groups. These positive trends can be attributed to the general recommendation since 1995 to vaccinate against hepatitis B. For hepatitis C, the prevalence of antibodies in the general population is 0.3 %. Germany thus remains a low-HCV-endemic country. An English full-text version of this article is available at SpringerLink as supplemental.

0 comments Cited 43 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

TORCH Infections. Toxoplasmosis, Other (syphilis, varicella-zoster, parvovirus B19), Rubella, Cytomegalovirus (CMV), and Herpes infections.

Barbara Stegmann, Chelsea J. Carey (2002)

Perinatal infections account for 2% to 3% of all congenital anomalies. TORCH, which includes Toxoplasmosis, Other (syphilis, varicella-zoster, parvovirus B19), Rubella, Cytomegalovirus (CMV), and Herpes infections, are some of the most common infections associated with congenital anomalies. Most of the TORCH infections cause mild maternal morbidity, but have serious fetal consequences, and treatment of maternal infection frequently has no impact on fetal outcome. Therefore, recognition of maternal disease and fetal monitoring once disease is recognized are important for all clinicians. Knowledge of these diseases will help the clinician appropriately counsel mothers on preventive measures to avoid these infections, and will aid in counseling parents on the potential for adverse fetal outcomes when these infections are present.

0 comments Cited 42 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Contributors

Xiaobo Yu: Role: Academic Editor

Joshua LaBaer: Role: Academic Editor

Journal

Journal ID (nlm-ta): High Throughput

Journal ID (iso-abbrev): High Throughput

Journal ID (publisher-id): high-throughput

Title: High-Throughput

Publisher: MDPI

ISSN (Electronic): 2571-5135

Publication date (Electronic): 30 October 2017

Publication date Collection: December 2017

Volume: 6

Issue: 4

Electronic Location Identifier: 14

Affiliations

[1 ]NMI Natural and Medical Sciences Institute at the University of Tuebingen, 72770 Reutlingen, Germany; angela.filomena@ 123456gmx.de (A.F.); thomas.joos@ 123456nmi.de (T.O.J.)

[2 ]TWINCORE, Centre for Experimental and Clinical Infection Research, 30625 Hanover, Germany; pessler.frank@ 123456mh-hannover.de (F.P.); manas.akmatov@ 123456helmholtz-hzi.de (M.K.A.); gerard.krause@ 123456helmholtz-hzi.de (G.K.)

[3 ]Helmholtz Centre for Infection Research, 38124 Brunswick, Germany

[4 ]Centre for Individualized Infection Medicine, 30625 Hanover, Germany

[5 ]Translational Infrastructure Epidemiology, German Centre for Infection Research (DZIF), 30625 Hanover, Germany

[6 ]Immunobiology of Dendritic Cells, Institute Pasteur, 75015 Paris, France; darragh.duffy@ 123456pasteur.fr (D.D.); matthew.albert@ 123456pasteur.fr (M.L.A.)

[7 ]Inserm U1223, Institute Pasteur, 75015 Paris, France

[8 ]Centre for Translational Research, Institute Pasteur, 75015 Paris, France

[9 ]Institut für Mikrobiologie und Hygiene, 66421 Homburg/Saar, Germany; barbara.gaertner@ 123456uks.eu

[10 ]TUM Technical University of Munich, 80333 Munich, Germany; markus.gerhard@ 123456tum.de

Author notes

[* ]Correspondence: nicole.schneiderhan@ 123456nmi.de ; Tel.: +49-(0)7121-51-530-815

Author information

Angela Filomena https://orcid.org/0000-0002-2301-4021

Manas K. Akmatov https://orcid.org/0000-0003-2310-3179

Markus Gerhard https://orcid.org/0000-0001-9110-3950

Article

Publisher ID: high-throughput-06-00014

DOI: 10.3390/ht6040014

PMC ID: 5748593

SO-VID: 0e8a974a-83cf-4328-a56d-6d941e83fbe8

License:

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

Development of a Bead-Based Multiplex Assay for the Analysis of the Serological Response against the Six Pathogens HAV, HBV, HCV, CMV, T. gondii, and H. pylori

Read this article at

Abstract

Related collections

When did Helicobacter first colonise humans?

Most cited references 22

Helicobacter pylori.

[Epidemiology of hepatitis A, B, and C among adults in Germany: results of the German Health Interview and Examination Survey for Adults (DEGS1)].

TORCH Infections. Toxoplasmosis, Other (syphilis, varicella-zoster, parvovirus B19), Rubella, Cytomegalovirus (CMV), and Herpes infections.

Author and article information

Contributors

Journal

Affiliations

Author notes

Author information

Article

History

Categories

Comments

Comment on this article

Similar content 114

Cited by 3

Most referenced authors 453