Distribution and Localization of Adenosine 3’:5’-Monophosphate-Dependent Protein Kinase in Mammalian Artery

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Abstract

Among the three major vascular layers (the intima-inner, the media-middle smooth muscle, and the adventitia-outer connective tissue) over 90% of the total protein kinase activity was observed in the middle layer. Of various subcellular fractions of the vascular smooth muscle, the 105,000 g supernatant (cytosol fraction) showed the highest specific activity and represented more than two thirds of the total kinase present in this layer. DEAE-cellulose chromatography of the soluble enzyme revealed the existence of two major forms of cyclic AMP-dependent protein kinase, type I and type II, of which 60% of the total enzymatic activity was found in type II. A divalent cation was found to be essential for their phosphotransferase activity. Only Mg2+ and Co2+, but not Zn2+, Mn2+ or Ca2+ could satisfy the cation requirement. The phosphorylated substrate had the characteristics of a protein with a phosphoester bond.

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Journal

Journal ID (publisher-id): JVR

Journal ID (nlm-ta): J Vasc Res

Journal ID (doi): 10.1159/issn.1018-1172

Title: Journal of Vascular Research

Publisher: S. Karger AG

ISSN (Print): 1018-1172

ISSN (Electronic): 1423-0135

Publication date Subscription-year: 1980

Publication date (Print): 1980

Publication date (Electronic): 19 September 2008

Volume: 17

Issue: 6

Pages: 312-323

Affiliations

Department of Anatomy and Cardiovasuclar Center, University of Iowa, Iowa City, Iowa

Article

Publisher ID: 158262 Other ID: Blood Vessels 1980;17:312–323

DOI: 10.1159/000158262

SO-VID: 0ed5f9d6-3242-4238-8caf-36314ea49ca3

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Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

History

Date received : 03 January 1980

Date accepted : 02 June 1980

Page count

Pages: 12

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