Blog
About

0
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found

      Distribution and Localization of Adenosine 3’:5’-Monophosphate-Dependent Protein Kinase in Mammalian Artery

      Read this article at

      ScienceOpenPublisher
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Among the three major vascular layers (the intima-inner, the media-middle smooth muscle, and the adventitia-outer connective tissue) over 90% of the total protein kinase activity was observed in the middle layer. Of various subcellular fractions of the vascular smooth muscle, the 105,000 g supernatant (cytosol fraction) showed the highest specific activity and represented more than two thirds of the total kinase present in this layer. DEAE-cellulose chromatography of the soluble enzyme revealed the existence of two major forms of cyclic AMP-dependent protein kinase, type I and type II, of which 60% of the total enzymatic activity was found in type II. A divalent cation was found to be essential for their phosphotransferase activity. Only Mg<sup>2+</sup> and Co<sup>2+</sup>, but not Zn<sup>2+</sup>, Mn<sup>2+</sup> or Ca<sup>2+</sup> could satisfy the cation requirement. The phosphorylated substrate had the characteristics of a protein with a phosphoester bond.

          Related collections

          Author and article information

          Journal
          JVR
          J Vasc Res
          10.1159/issn.1018-1172
          Journal of Vascular Research
          S. Karger AG
          1018-1172
          1423-0135
          1980
          1980
          19 September 2008
          : 17
          : 6
          : 312-323
          Affiliations
          Department of Anatomy and Cardiovasuclar Center, University of Iowa, Iowa City, Iowa
          Article
          158262 Blood Vessels 1980;17:312–323
          10.1159/000158262
          © 1980 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 12
          Categories
          Research Paper

          Comments

          Comment on this article