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      Distribution and Localization of Adenosine 3’:5’-Monophosphate-Dependent Protein Kinase in Mammalian Artery

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          Among the three major vascular layers (the intima-inner, the media-middle smooth muscle, and the adventitia-outer connective tissue) over 90% of the total protein kinase activity was observed in the middle layer. Of various subcellular fractions of the vascular smooth muscle, the 105,000 g supernatant (cytosol fraction) showed the highest specific activity and represented more than two thirds of the total kinase present in this layer. DEAE-cellulose chromatography of the soluble enzyme revealed the existence of two major forms of cyclic AMP-dependent protein kinase, type I and type II, of which 60% of the total enzymatic activity was found in type II. A divalent cation was found to be essential for their phosphotransferase activity. Only Mg<sup>2+</sup> and Co<sup>2+</sup>, but not Zn<sup>2+</sup>, Mn<sup>2+</sup> or Ca<sup>2+</sup> could satisfy the cation requirement. The phosphorylated substrate had the characteristics of a protein with a phosphoester bond.

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          Author and article information

          J Vasc Res
          Journal of Vascular Research
          S. Karger AG
          19 September 2008
          : 17
          : 6
          : 312-323
          Department of Anatomy and Cardiovasuclar Center, University of Iowa, Iowa City, Iowa
          158262 Blood Vessels 1980;17:312–323
          © 1980 S. Karger AG, Basel

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          Pages: 12
          Research Paper


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