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      An instrumental set up for seed germination studies with temperature control and automatic image recording Translated title: Dispositivo para estudos de germinação com controle de temperatura e registro automático de imagem

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          Abstract

          This paper describes a new instrumental methodology proposed for the study of seed germination which can be applied to germination physiology, agriculture and forest restoration. This instrument performs the recording of images of germinating seeds through the use of a flat-bed scanner coupled to a computer, where a specific program commands the capture of these images at predetermined time intervals. These images are archived for posterior analysis. The device allows the collection of data and the elaboration of germination curves with high accuracy. Furthermore, the device permits the study of seed germination over a broad range of temperatures as well as the easy application of chemical agents to the seeds in order to study their influence on the germination process. Lactuca sativa seeds were used in this study as an experimental model. Germination curves were plotted at different temperatures in order to evaluate the device for experimental work. This paper also presents a methodology of statistical analysis that supports the reliability of this equipment.

          Translated abstract

          Este artigo descreve uma nova metodologia instrumental para o estudo de germinação de sementes aplicável à fisiologia da germinação, agricultura e a restauração de florestas. Este dispositivo registra imagens de sementes em germinação mediante o uso de um "scanner" acoplado a um computador, onde um programa específico comanda a captura dessas imagens para intervalos de tempo pré-determinados. Estas imagens são arquivadas para posterior análise. Este dispositivo permite a coleção de dados para a elaboração de curvas de germinação de modo particularmente acurado, em que a germinação das sementes pode ser realizada para uma larga faixa de temperatura; além disso, abre-se a possibilidade de aplicação fácil de agentes químicos nas sementes, no sentido de estudar a influência desses agentes no processo de germinação. Sementes de Lactuca sativa foram usadas como modelo experimental. Foram estabelecidas curvas de germinação para diferentes temperaturas, no sentido de avaliar nosso dispositivo em trabalho efetivo. Este artigo apresenta também uma metodologia de análise estatística que garante a confiabilidade do dispositivo ora descrito.

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          Activation of gibberellin biosynthesis and response pathways by low temperature during imbibition of Arabidopsis thaliana seeds.

          Exposure of imbibed seeds to low temperature (typically 4 degrees C) is widely used to break seed dormancy and to improve the frequency of germination. However, the mechanism by which temperature accelerates germination is largely unknown. Using DNA microarray and gas chromatography-mass spectrometry analyses, we found that a subset of gibberellin (GA) biosynthesis genes were upregulated in response to low temperature, resulting in an increase in the level of bioactive GAs and transcript abundance of GA-inducible genes in imbibed Arabidopsis thaliana seeds. Using a loss-of-function mutant, the cold-inducible GA biosynthesis gene, AtGA3ox1, was shown to play an essential role in mediating the effect of low temperature. Besides temperature, AtGA3ox1 also is positively regulated by active phytochrome and negatively regulated by GA activity. We show that both red light and GA deficiency act in addition to low temperature to elevate the level of AtGA3ox1 transcript, indicating that multiple signals are integrated by the AtGA3ox1 gene to control seed germination. When induced by low temperature, AtGA3ox1 mRNA was detectable by in situ RNA hybridization in an additional set of cell types relative to that in red light-induced seeds. Our results illustrate that the GA biosynthesis and response pathways are activated during seed imbibition at low temperature and suggest that the cellular distribution of bioactive GAs may be altered under different light and temperature conditions.
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            BioEstat 5.3: Aplicações estatísticas nas áreas das ciências biológicas e médicas

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              The application of image analysis in monitoring the imbibition process of white cabbage (Brassica oleraceae) seeds

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                bjpp
                Brazilian Journal of Plant Physiology
                Braz. J. Plant Physiol.
                Brazilian Journal of Plant Physiology (Campos dos Goytacazes )
                1677-9452
                June 2007
                : 19
                : 2
                : 99-108
                Affiliations
                [1 ] Fundação Mokiti Okada Brasil
                Article
                S1677-04202007000200002
                10.1590/S1677-04202007000200002
                0ef44452-a17f-441c-8083-2972787e5f7b

                http://creativecommons.org/licenses/by/4.0/

                History
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                SciELO Brazil

                Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=1677-0420&lng=en
                Categories
                PLANT SCIENCES

                Plant science & Botany
                digital image,Lactuca sativa,scanner,Siegel's test,thermal gradient tables,imagem digital,mesa de gradiente térmico,"scanner",teste de Siegel

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