Application of UPT-POCT in Public Health Emergencies

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Abstract

Rapid and reliable detection of infectious agents on site is essential for timely initiation of medical treatment and post-exposure prophylactic measures when public health emergencies occur. However, the referee standard for confirmation of infectious agents remains laboratory diagnosis, which is time-consuming and not available in the field. UPT-POCT technology is a versatile tool that requires limited resources and can realize rapid detection of infectious agents on site, providing timely information for the quick response to public health emergencies.

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Point-of-care testing (POCT): Current techniques and future perspectives

Peter Luppa, Carolin Müller, Alice Schlichtiger … (2011)

Point-of-care testing (POCT) is a laboratory-medicine discipline that is evolving rapidly in analytical scope and clinical application. In this review, we first describe the state of the art of medical-laboratory tests that can be performed near the patient. At present, POCT ranges from basic blood-glucose measurement to complex viscoelastic coagulation assays. POCT shortens the time to clinical decision-making about additional testing or therapy, as delays are no longer caused by transport and preparation of clinical samples, and biochemical-test results are rapidly available at the point of care. Improved medical outcome and lower costs may ensue. Recent, evolving technological advances enable the development of novel POCT instruments. We review the underlying analytical techniques. If new instruments are not yet in practical use, it is often hard to decide whether the underlying analytical principle has real advantage over former methods. However, future utilization of POCT also depends on health-care trends and new areas of application. But, even today, it can be assumed that, for certain applications, near-patient testing is a useful complement to conventional laboratory analyses.

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Development of a lateral flow colloidal gold immunoassay strip for the simultaneous detection of Shigella boydii and Escherichia coli O157:H7 in bread, milk and jelly samples

Chunmei Song, Cheng Liu, Shuyan Wu … (2016)

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Comparison of culture, ELISA and PCR techniques for salmonella detection in faecal samples for cattle, pig and poultry

Erik Eriksson, Anna Aspan (2007)

Background Performances of different salmonella detection methods were evaluated by applying them to of artificially contaminated faecal specimens from cattle, pigs and poultry. The NMKL71 method, being the standard reference method for detection of salmonella in the official Swedish control program, was compared with the proposed ISO method using MSRV-selective enrichment for culturing, and also with three commercial ELISA- based systems, Bioline Selecta, Bioline Optima and Vidas, a commercial PCR-based method, BAX® system, and three different strategies using PCR detection using a non-commercial PCR system. Results Altogether, 391 samples were tested, and the overall results clearly indicate that, when faeces from all animal species and all serotypes were included, the MSRV performed best, with a calculated accuracy of 99% and a calculated sensitivity of 98%. The second most sensitive and specific method was the BAX® system, using the modified enrichment protocol as recommended by the manufacturer for faecal samples. However, this protocol includes one additional day of work, as compared with the standard procedure for food sample analysis by the same method. The different strategies for salmonella detection using non-commercial PCR showed a sensitivity and specificity in the same range as the BAX® method; furthermore, results were obtained more quickly. The various commercial ELISA methods and the NMKL method showed the poorest performance of the methods included in the study, and were closely dependent on the origin of the faeces used and on which salmonella strain was to be detected. Conclusion The study showed that the sensitivity of the different methods depended to a great extent on the origin of the faecal matrices and the salmonella strains used to "spike" the samples.

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Author and article information

Contributors

Ruifu Yang: 13801034560@163.com

Yong Zhao: zhaoyong179@139.com

Journal

Journal ID (publisher-id): 978-981-32-9279-6

Journal ID (doi): 10.1007/978-981-32-9279-6

Journal ID (nlm-ta): Principles and Applications of Up-converting Phosphor Technology

Title: Principles and Applications of Up-converting Phosphor Technology

ISBN (Print): 978-981-32-9278-9

ISBN (Electronic): 978-981-32-9279-6

Publication date (Electronic): 20 September 2019

Pages: 187-190

Affiliations

GRID grid.198530.6, ISNI 0000 0000 8803 2373, State Key Laboratory of Pathogen and Biosecurity, , Beijing Institute of Microbiology and Epidemiology, ; Beijing, China

[2 ]Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing, 100071 People’s Republic of China

[3 ]Beijing Hotgen Biotechnology Co., Ltd., Beijing Z-Park Daxing Biomedical Industry Base, No. 9, Tianfujie, Beijing, 102600 China

[4 ]Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, No. 20, Dongdajie, Fengtai District, Beijing, 100071 China

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Publisher ID: 13

DOI: 10.1007/978-981-32-9279-6_13

PMC ID: 7120900

SO-VID: 0ef6dae0-7861-4723-94ab-fbd03a025dc3

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This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.

Application of UPT-POCT in Public Health Emergencies

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Zika Virus

Most cited references 10

Point-of-care testing (POCT): Current techniques and future perspectives

Development of a lateral flow colloidal gold immunoassay strip for the simultaneous detection of Shigella boydii and Escherichia coli O157:H7 in bread, milk and jelly samples

Comparison of culture, ELISA and PCR techniques for salmonella detection in faecal samples for cattle, pig and poultry

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