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      Next-generation genetic code expansion

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      Current Opinion in Chemical Biology
      Elsevier BV

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          Abstract

          <p class="first" id="P2">Engineering of the translation apparatus has permitted the site-specific incorporation of nonstandard amino acids (nsAAs) into proteins, thereby expanding the genetic code of organisms. Conventional approaches have focused on porting tRNAs and aminoacyl-tRNA synthetases (aaRS) from archaea into bacterial and eukaryotic systems where they have been engineered to site-specifically encode nsAAs. More recent work in genome engineering has opened up the possibilities of whole genome recoding, in which organisms with alternative genetic codes have been constructed whereby codons removed from the genetic code can be repurposed as new sense codons dedicated for incorporation of nsAAs. These advances, together with the advent of engineered ribosomes and new molecular evolution methods, enable multisite incorporation of nsAAs and unnatural monomers paving the way for the template-directed production of functionalized proteins, new classes of polymers, and genetically encoded materials. </p>

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          Author and article information

          Journal
          Current Opinion in Chemical Biology
          Current Opinion in Chemical Biology
          Elsevier BV
          13675931
          July 2018
          July 2018
          Article
          10.1016/j.cbpa.2018.07.020
          6748649
          30072242
          0efb7205-1926-4d00-a961-024cb1894da0
          © 2018

          https://www.elsevier.com/tdm/userlicense/1.0/

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