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      Three-dimensional engineered heart tissue from neonatal rat cardiac myocytes

      , , , , ,
      Biotechnology and Bioengineering
      Wiley

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          Abstract

          A technique is presented that allows neonatal rat cardiac myocytes to form spontaneously and coherently beating 3-dimensional engineered heart tissue (EHT) in vitro, either as a plane biconcaval matrix anchored at both sides on Velcro-coated silicone tubes or as a ring. Contractile activity was monitored in standard organ baths or continuously in a CO(2) incubator for up to 18 days (=26 days after casting). Long-term measurements showed an increase in force between days 8 and 18 after casting and stable forces thereafter. At day 10, the twitch amplitude (TA) of electrically paced EHTs (average length x width x thickness, 11 x 6 x 0.4 mm) was 0.51 mN at length of maximal force development (L(max)) and a maximally effective calcium concentration. EHTs showed typical features of neonatal rat heart: a positive force-length and a negative force-frequency relation, high sensitivity to calcium (EC(50) 0.24 mM), modest positive inotropic (increase in TA by 46%) and pronounced positive lusitropic effect of isoprenaline (decrease in twitch duration by 21%). Both effects of isoprenaline were sensitive to the muscarinic receptor agonist carbachol in a pertussis toxin-sensitive manner. Adenovirus-mediated gene transfer of beta-galactosidase into EHTs reached 100% efficiency. In summary, EHTs retain many of the physiological characteristics of rat cardiac tissue and allow efficient gene transfer with subsequent force measurement. Copyright 2000 John Wiley & Sons, Inc.

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          Author and article information

          Journal
          Biotechnology and Bioengineering
          Biotechnol. Bioeng.
          Wiley
          0006-3592
          1097-0290
          April 05 2000
          April 05 2000
          : 68
          : 1
          : 106-114
          Article
          10.1002/(SICI)1097-0290(20000405)68:1<106::AID-BIT13>3.0.CO;2-3
          10699878
          0f0f1fda-2950-47fe-b091-8398a651dfa5
          © 2000

          http://doi.wiley.com/10.1002/tdm_license_1.1

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