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      Growth Factors Induce Actin Disruption in Cultured Human Retinal Pigment Epithelial Cells

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          Exposure of cultured human retinal pigment epithelial cells to platelet-derived growth factor, nerve growth factor or epidermal growth factor resulted in a time- and dose-dependent alteration in the distribution of actin stained by rhodamine-phalloidin. These growth factors (platelet-derived growth factor of 80 ng/ml, nerve growth factor of 10 ng/ml or epidermal growth factor of 10 ng/ml) caused disappearance of actin filaments from the peripheral region of a cell in 1 or 2 h and change of cell configuration to spindle shape in 3 or 4 h. Other growth factors, fibroblast growth factor of 10 ng/ml and insulin of 25 μmol/ml had no effect on actin distribution. The alteration of actin and the change of cellular shape might be associated with stimulation of cell growth and migration of retinal pigment epithelial cells.

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          Author and article information

          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          04 December 2009
          : 18
          : 6
          : 338-342
          Laboratory of Ocular Cell Biology, Department of Ophthalmology, Kyoto University Faculty of Medicine, Kyoto, Japan
          265460 Ophthalmic Res 1986;18:338–342
          © 1986 S. Karger AG, Basel

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          Pages: 5
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