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      Characterization of Bacterial Community, Ammonia-Oxidizing Bacteria, and Nitrospira During the Operation of a Commercial-Scale Recirculating Aquaculture System for Culturing Pufferfish Takifugu rubripes

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          Abstract

          We investigated the changes in communities of bacteria, ammonia-oxidizing bacteria, and Nitrospira during the operation of a pufferfish Takifugu rubripes recirculating aquaculture system by using high-throughput DNA sequencing. Differences in bacterial communities were observed at days 1 – 32, 47 – 62 and 78 – 93 of biofilm development by using 16S rRNA gene pyrosequencing. The relative abundance of Proteobacteria (Gammaproteobacteria) increased, while that of Bacteroidetes (Flavobacteria) decreased. The proportions of Nitrosomonas and Nitrospina ranged from 0.02% to 0.30% and from 0.02% to 0.83%, respectively. Ammonia monooxygenase gene pyrosequencing revealed that the top three operational taxonomic units were related to Nitrosomonas aestuarii (17.5% – 61.1%), uncultured beta proteobacterium clone B67S-54 (1.9% – 45.2%), and uncultured bacterium clone AZPa8 (3.6% – 24.7%). Nitrite oxidoreductase gene pyrosequencing revealed that the relative abundance of the dominant strain Nitrospira sp. Ecomares 2.1 increased, but that of the abundant species Nitrospira marina decreased. Our results demonstrated that the communities of bacteria, ammonia-oxidizing bacteria, and Nitrospira were changing during the operation of the pufferfish recirculating aquaculture system.

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          Author and article information

          Journal
          JOUC
          Journal of Ocean University of China
          Science Press and Springer (China )
          1672-5182
          15 November 2020
          01 December 2020
          : 19
          : 6
          : 1399-1408
          Affiliations
          1Key Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian 116023, China
          2Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China
          3Dalian Tianzheng Industry Company Limited, Dalian 116011, China
          4College of Environmental Science and Engineering, Dalian Maritime University, Dalian 116026, China
          Author notes
          *Corresponding author: MA Yuexin, Tel: 0086-411-84763096, E-mail: mayuexin@ 123456dlou.edu.cn
          Article
          s11802-020-4472-7
          10.1007/s11802-020-4472-7
          Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2020.

          The copyright to this article, including any graphic elements therein (e.g. illustrations, charts, moving images), is hereby assigned for good and valuable consideration to the editorial office of Journal of Ocean University of China, Science Press and Springer effective if and when the article is accepted for publication and to the extent assignable if assignability is restricted for by applicable law or regulations (e.g. for U.S. government or crown employees).

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          Self URI (journal-page): https://www.springer.com/journal/11802

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