39
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Impacts of Horticultural Mineral Oils and Two Insecticide Practices on Population Fluctuation of Diaphorina citri and Spread of Huanglongbing in a Citrus Orchard in Sarawak

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Aspects of the incidence and spread of the citrus disease huanglongbing (HLB) in relation to the vector Diaphorina citri population fluctuation were studied from January 1999 to December 2001 seasons in a 0.8 ha citrus orchard at Jemukan (1° 33′N, 110° 41′E), Southwest Sarawak in Malaysia. In relation to insecticide and horticultural mineral oils (HMOs) use, levels of HLB infection rose quite rapidly over the next 3 years in the unsprayed control and less rapidly in the other treatments such as imidacloprid, nC24HMO, and triazophos/cypermethrin/chlorpyrifos. Levels of HLB as determined by Polymerase Chain Reaction (PCR) were 42.2%, 9.4%, 11.4%, and 22.7%, respectively. The effects of nC 24HMO and conventional pesticides on the citrus psyllid population and parasitoids in citrus orchard were also determined.

          Related collections

          Most cited references75

          • Record: found
          • Abstract: found
          • Article: not found

          The phloem-limited bacterium of greening disease of citrus is a member of the alpha subdivision of the Proteobacteria.

          Using the PCR, we amplified the 16S ribosomal DNAs (rDNAs) of an Asian strain and an African strain of the uncultured, gram-negative, walled, phloem-limited bacterium-like organism (BLO) associated with citrus greening disease. We evaded coamplification of chloroplast 16S rDNA by using restriction enzymes; the chloroplast 16S rDNA was sensitive to BclI digestion and resistant to EcoRI digestion, while the 16S rDNA of the BLO was resistant to BclI digestion and sensitive to EcoRI digestion. The 16S rDNA of the African BLO strain was amplified from BclI-digested DNA extracted from infected periwinkle leaf midribs. The Asian strain was isolated from plant extract by using a specific monoclonal antibody coated onto the surface of a PCR tube. The 16S rDNAs of the two BLO strains were cloned and sequenced. Comparisons with sequences of 16S rDNAs obtained from the GenBank data base revealed that the two citrus greening disease BLOs belong to the alpha subdivision of the class Proteobacteria. Even though their closest relatives are members of the alpha-2 subgroup, these BLOs are distinct from this subgroup as we observed only 87.5% homology between the 16S rDNAs examined. Therefore, the two BLOs which we studied probably are members of a new lineage in the alpha subdivision of the Proteobacteria. We propose the trivial name "liberobacter" for this new group of bacteria and will wait until additional characteristics have been determined before we propose a formal name.
            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            Citrus Greening Disease

            J Graca (1991)
              Bookmark
              • Record: found
              • Abstract: not found
              • Article: not found

              The Neotropical Psylloidea (Homoptera: Insecta): an annotated check list

                Bookmark

                Author and article information

                Journal
                ScientificWorldJournal
                ScientificWorldJournal
                TSWJ
                The Scientific World Journal
                The Scientific World Journal
                1537-744X
                2012
                2 May 2012
                : 2012
                : 651416
                Affiliations
                1Faculty of Agriculture and Food Sciences, Universiti Putra Malaysia Campus Bintulu Sarawak, Jalan Nyabau, 97008 Bintulu, Sarawak, Malaysia
                2Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
                3Centre for Plant and Food Science, University of Western Sydney, Locked Bag 1797, Penrith New South Wales 2751, Australia
                Author notes
                *Stephen Chan Teck Leong: sctleong@ 123456btu.upm.edu.my

                Academic Editor: Aurelio Gómez-Cadenas

                Article
                10.1100/2012/651416
                3354681
                22629178
                0f3b9a4e-7717-4acf-b1c0-4a38f2b8520a
                Copyright © 2012 Stephen Chan Teck Leong et al.

                This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                : 11 November 2011
                : 1 February 2012
                Categories
                Research Article

                Uncategorized
                Uncategorized

                Comments

                Comment on this article