Abnormal Karyotype of Pen Shell ( Atrina pectinata) During Its Early Embryonic Development in Late Breeding Season

Abstract

The pen shell, Atrina pectinata, distributes globally. It is one of the most important edible bivalves in east Asian countries. However,there are multiple difficulties in rearing pen shell larvae and juveniles because of their high mortality. To understand the mechanism underlining such high mortality at the early embryonic development stage, we obtained approximately 100 million larvae during later breeding season in June, 2017, and checked a large amount of mitotic chromosomal plates of the early embryos and post-spawning gonads tissue slices of their parents. The results showed that most diploid A. pectinata embryos have 17 pairs of chromosomes ( 2n = 34) as their parents do. The first pair of particularly large chromosomes are heterotypic in some diploid embryos while they are homomorphic in others. The primary sex-determination chromosome type is XX/XY. A lot of triploid, pentaploid and aneuploid embryos with different numbers of the largest homomorphic or heteromorphic chromosomes were found due to the degeneration of overmatured parent gonads which hold normal karyotype. These larvae will die even though most of them may develop into the trochophore stage with 34 chromosomes. Genetic deficiency of chromosomes will cause a high rate of mortality in early embryos in late breeding season. These findings should enrich the current knowledge of juvenile pen shell aquaculture.

Journal

Journal ID (publisher-id): JOUC

Title: Journal of Ocean University of China

Publisher: Science Press and Springer (China )

ISSN (Electronic): 1672-5182

Publication date (Electronic): 10 July 2020

Publication date (Print): 01 August 2020

Volume: 19

Issue: 4

Pages: 902-910

Affiliations

¹Key Laboratory for Sustainable Development of Marine Fisheries of Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Qingdao 266071, China

²Laboratory for Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China

³Rizhao Ocean and Fisheries Research Institute, Rizhao 276826, China

⁴College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

Author notes

*Corresponding author: YANG Aiguo, E-mail: yangag@ 123456ysfri.ac.cn

Article

Publisher ID: s11802-020-4280-0

DOI: 10.1007/s11802-020-4280-0

Copyright statement: Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2020.

License:

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Product

Self URI (journal-page): https://www.springer.com/journal/11802