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      Down-regulation of CD53 expression in Epinephelus coioides under LPS, poly (I:C), and cytokine stimulation.

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          Abstract

          Tetraspanins are a group of cell surface molecules involved in cell adhesion, motility, metastasis, signal transduction, and immune cell activation. Members of the tetraspanin family include CD9, CD37, CD63, CD53, and others. However, few tetraspanins have been investigated in teleosts. In this study, we obtained the open reading frame of CD53 cDNA from orange spotted grouper (Epinephelus coioices), an economically important fish. The predicted amino acid structure contains four membrane-spanning domains and a conserved CCG motif. The amino acid identity between human and grouper CD53 was only 38%; however, both CD53 proteins share the same structure. Quantitative real-time PCR revealed that mRNA is abundant in immune organs, including the head and trunk kidneys, spleen, thymus, gill, and blood. Immunochemistry and immunofluorescence analyses further revealed that CD53 was majorly expressed in the leukocytes of various organs. Finally, mRNA and protein expression for CD53 was down-regulated in fish treated with immune stimulators, including LPS, Poly (I:C), Vibrio, recombinant grouper IL-6, and CCL4. Our results indicate that the expression of CD53 may play important roles in pathogen invasion and inflammation reaction.

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          Author and article information

          Journal
          Fish Shellfish Immunol.
          Fish & shellfish immunology
          Elsevier BV
          1095-9947
          1050-4648
          Apr 2016
          : 51
          Affiliations
          [1 ] Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan; Chi Mei Medical Center, Liouying, No.201, Taikang, Liuying Dist., Tainan City 736, Taiwan.
          [2 ] Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan; Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan 701, Taiwan; Research Center of Agricultural Biotechnology, National Cheng Kung University, Tainan 701, Taiwan; Center of Biosciences, National Cheng Kung University, Tainan 701, Taiwan.
          [3 ] Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
          [4 ] Department of Biotechnology, Ming Chuan University, Taoyuan County 333, Taiwan. Electronic address: htlin@mail.mcu.edu.tw.
          Article
          S1050-4648(15)30251-5
          10.1016/j.fsi.2015.11.032
          26631805
          0f7e0e3a-fd27-4345-a89c-6c25f85b695f
          History

          Tetraspanin,Poly (I:C),LPS,Epinephelus coioides,CD53
          Tetraspanin, Poly (I:C), LPS, Epinephelus coioides, CD53

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