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      Effects of Nitrogen Supply on Water Stress and Recovery Mechanisms in Kentucky Bluegrass Plants

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          Abstract

          Non-irrigated crops in temperate and irrigated crops in arid regions are exposed to an incessant series of drought stress and re-watering. Hence, quick and efficient recuperation from drought stress may be amongst the key determinants of plant drought adjustment. Efficient nitrogen (N) nutrition has the capability to assuage water stress in crops by sustaining metabolic activities even at reduced tissue water potential. This study was designed to understand the potential of proper nutrition management by studying the morphological and physiological attributes, and assimilation of nitrogen in Kentucky bluegrass under drought stress. In present study, one heterogeneous habitat and four treatments homogenous habitats each with four replications were examined during field trial. Drought stress resulted in a significant reduction in the nitrogen content of both mother and first ramets, maximum radius, above and below ground mass, number of ramets per plot, leaf water contents and water potential and increased the carbon content and the C:N ratio in both homogenous and heterogeneous plots compared to well-watered and nutritional conditions. Observation using electron microscopy showed that drought stress shrunk the vessel diameter, circumference and xylem area, but increased the sieve diameter, and phloem area in the leaf crosscutting structure of Kentucky bluegrass, first, second, and third ramet leaf. Thus, it can be concluded that water stress markedly reduced all the important traits of Kentucky bluegrass, however, proper nutritional management treatment resulted in the best compensatory performance under drought assuaging its adversity up to some extent and may be considered in formulating good feasible and cost-effective practices for the environmental circumstances related to those of this study.

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          Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell.

          Plants are often subjected to periods of soil and atmospheric water deficits during their life cycle as well as, in many areas of the globe, to high soil salinity. Understanding how plants respond to drought, salt and co-occurring stresses can play a major role in stabilizing crop performance under drought and saline conditions and in the protection of natural vegetation. Photosynthesis, together with cell growth, is among the primary processes to be affected by water or salt stress. The effects of drought and salt stresses on photosynthesis are either direct (as the diffusion limitations through the stomata and the mesophyll and the alterations in photosynthetic metabolism) or secondary, such as the oxidative stress arising from the superimposition of multiple stresses. The carbon balance of a plant during a period of salt/water stress and recovery may depend as much on the velocity and degree of photosynthetic recovery, as it depends on the degree and velocity of photosynthesis decline during water depletion. Current knowledge about physiological limitations to photosynthetic recovery after different intensities of water and salt stress is still scarce. From the large amount of data available on transcript-profiling studies in plants subjected to drought and salt it is becoming apparent that plants perceive and respond to these stresses by quickly altering gene expression in parallel with physiological and biochemical alterations; this occurs even under mild to moderate stress conditions. From a recent comprehensive study that compared salt and drought stress it is apparent that both stresses led to down-regulation of some photosynthetic genes, with most of the changes being small (ratio threshold lower than 1) possibly reflecting the mild stress imposed. When compared with drought, salt stress affected more genes and more intensely, possibly reflecting the combined effects of dehydration and osmotic stress in salt-stressed plants.
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            Plant drought stress: effects, mechanisms and management

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              Understanding plant responses to drought — from genes to the whole plant

              Functional Plant Biology, 30(3), 239 In the last decade, our understanding of the processes underlying plant response to drought, at the molecular and whole-plant levels, has rapidly progressed. Here, we review that progress. We draw attention to the perception and signalling processes (chemical and hydraulic) of water deficits. Knowledge of these processes is essential for a holistic understanding of plant resistance to stress, which is needed to improve crop management and breeding techniques. Hundreds of genes that are induced under drought have been identified. A range of tools, from gene expression patterns to the use of transgenic plants, is being used to study the specific function of these genes and their role in plant acclimation or adaptation to water deficit. However, because plant responses to stress are complex, the functions of many of the genes are still unknown. Many of the traits that explain plant adaptation to drought — such as phenology, root size and depth, hydraulic conductivity and the storage of reserves — are those associated with plant development and structure, and are constitutive rather than stress induced. But a large part of plant resistance to drought is the ability to get rid of excess radiation, a concomitant stress under natural conditions. The nature of the mechanisms responsible for leaf photoprotection, especially those related to thermal dissipation, and oxidative stress are being actively researched. The new tools that operate at molecular, plant and ecosystem levels are revolutionising our understanding of plant response to drought, and our ability to monitor it. Techniques such as genome-wide tools, proteomics, stable isotopes and thermal or fluorescence imaging may allow the genotype–phenotype gap to be bridged, which is essential for faster progress in stress biology research.
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                Author and article information

                Contributors
                Journal
                Front Plant Sci
                Front Plant Sci
                Front. Plant Sci.
                Frontiers in Plant Science
                Frontiers Media S.A.
                1664-462X
                08 June 2017
                2017
                : 8
                : 983
                Affiliations
                [1] 1College of Horticulture, Northeast Agricultural University Harbin, China
                [2] 2College of Plant Science and Technology, Huazhong Agricultural University Wuhan, China
                [3] 3Cholistan Institute of Desert Studies, The Islamia University of Bahawalpur Bahawalpur, Pakistan
                [4] 4Department of Agronomy, The Islamia University of Bahawalpur Bahawalpur, Pakistan
                [5] 5Department of Environmental Sciences, COMSATS Institute of Information Technology Vehari, Pakistan
                [6] 6Department of Agronomy, Faculty of Crop Production Sciences, The University of Agriculture Peshawar, Pakistan
                [7] 7Department of Biological Sciences, Faculty of Science, King Abdulaziz University Jeddah, Saudi Arabia
                Author notes

                Edited by: Xunzhong Zhang, Virginia Tech, United States

                Reviewed by: Carmen Arena, University of Naples Federico II, Italy; Mitsutoshi Kitao, Forestry and Forest Products Research Institute, Japan

                *Correspondence: Chen Yajun, chenyajun622@ 123456163.com

                These authors have contributed equally to this work.

                This article was submitted to Plant Abiotic Stress, a section of the journal Frontiers in Plant Science

                Article
                10.3389/fpls.2017.00983
                5463276
                28642781
                0fb01147-78c9-4f60-b4ea-41b8cc33d0ad
                Copyright © 2017 Saud, Fahad, Yajun, Ihsan, Hammad, Nasim, Amanullah, Arif and Alharby.

                This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

                History
                : 05 February 2017
                : 24 May 2017
                Page count
                Figures: 11, Tables: 4, Equations: 0, References: 74, Pages: 18, Words: 0
                Funding
                Funded by: National Natural Science Foundation of China 10.13039/501100001809
                Categories
                Plant Science
                Original Research

                Plant science & Botany
                kentucky bluegrass,drought,nutritional soil,transmission electron microscopy,c:n ratio,homogenous and heterogeneous plots

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