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      Effect of polypeptide from Chlamys farreri on UVB-induced ROS/NF-κB/COX-2 activation and apoptosis in HaCaT cells

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      Journal of Photochemistry and Photobiology B: Biology
      Elsevier BV

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          Abstract

          Polypeptide from Chlamys farreri (PCF) is a novel marine polypeptide compound isolated from gonochoric Chinese scallop Chlamys farreri, this study we further investigate the mechanisms of PCF exerting its anti-apoptotic effect. The results indicated that PCF, ROS scavenger NAC and NF-kappaB inhibitor MG132 effectively inhibited UVB-induced HaCaT cells apoptosis. PCF (2.84mM) showed potential ROS scavenging activities in a kinetic process. PCF (1.42-5.69mM) dose-dependently increased the expressions of Cu, Zn-SOD, CAT and GPx meanwhile decreased the expressions of p-NF-kappaB/p65 and COX-2 in UVB-induced HaCaT cells. Additionally, pretreatment with NAC significantly declined the generation of ROS and the expression of p-NF-kappaB/p65. We concluded that ROS, NF-kappaB and COX-2 are involved in UVB-induced HaCaT cells apoptosis, PCF exerts its protective effects via scavenging ROS, increasing the expression of antioxidative enzymes and inhibition the activation of NF-kappaB and COX-2.

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          Author and article information

          Journal
          Journal of Photochemistry and Photobiology B: Biology
          Journal of Photochemistry and Photobiology B: Biology
          Elsevier BV
          10111344
          August 2009
          August 2009
          : 96
          : 2
          : 109-116
          Article
          10.1016/j.jphotobiol.2009.04.010
          19481953
          0fd92a34-0957-4a3a-bd7a-f4a435b8002c
          © 2009

          https://www.elsevier.com/tdm/userlicense/1.0/

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