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      Soft X-ray diffraction microscopy of a frozen hydrated yeast cell.

      Physical review letters
      Freezing, Microscopy, methods, Saccharomyces cerevisiae, cytology, Water, metabolism, X-Ray Diffraction

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          Abstract

          We report the first image of an intact, frozen hydrated eukaryotic cell using x-ray diffraction microscopy, or coherent x-ray diffraction imaging. By plunge freezing the specimen in liquid ethane and maintaining it below -170 degrees C, artifacts due to dehydration, ice crystallization, and radiation damage are greatly reduced. In this example, coherent diffraction data using 520 eV x rays were recorded and reconstructed to reveal a budding yeast cell at a resolution better than 25 nm. This demonstration represents an important step towards high resolution imaging of cells in their natural, hydrated state, without limitations imposed by x-ray optics.

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          Author and article information

          Journal
          20365955
          2866741
          10.1103/PhysRevLett.103.198101

          Chemistry
          Freezing,Microscopy,methods,Saccharomyces cerevisiae,cytology,Water,metabolism,X-Ray Diffraction
          Chemistry
          Freezing, Microscopy, methods, Saccharomyces cerevisiae, cytology, Water, metabolism, X-Ray Diffraction

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