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      Identification of Microplankton in South China Sea with Image-Matching Individual PCR

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          Abstract

          In this study, marine microplankton were identified by combining standard light microscopy with Sanger 18S rRNA gene sequencing. The image-matching individual PCR technique was applied to identify the image collectable unicellular microplankton to genera. Instead of pure strain culture and morphological identification, microplankton individual cells were isolated and fixed with glutaraldehyde, frozen and stored for months. Finally, they were imaged under a microscope and molecularly identified via phylogenetic analysis of their 18S ribosomal RNA gene (18S rDNA). Microplankton cells were collected at 30 locations in South China Sea, and were assigned to 21 known and 4 unidentified genera (2 uncultured fungi and 2 uncultured stramenopiles) with phylogenetic analysis in parallel to the morphological identification.

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          Author and article information

          Journal
          JOUC
          Journal of Ocean University of China
          Science Press and Springer (China )
          1672-5182
          10 January 2019
          01 February 2019
          : 18
          : 1
          : 219-226
          Affiliations
          1 School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China
          2 Engineering Research Center of Seawater Utilization Technology of Chinese Ministry of Education, Hebei University of Technology, Tianjin 300130, China
          3 College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
          4 Key Laboratory of Mariculture of Chinese Ministry of Education, Ocean University of China, Qingdao 266003, China
          Author notes
          *Corresponding author: PAN Kehou
          Article
          s11802-019-3746-4
          10.1007/s11802-019-3746-4
          Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2019.

          The copyright to this article, including any graphic elements therein (e.g. illustrations, charts, moving images), is hereby assigned for good and valuable consideration to the editorial office of Journal of Ocean University of China, Science Press and Springer effective if and when the article is accepted for publication and to the extent assignable if assignability is restricted for by applicable law or regulations (e.g. for U.S. government or crown employees).

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          Self URI (journal-page): https://www.springer.com/journal/11802

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