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      Relationships among superantigen toxin gene profiles, genotypes, and pathogenic characteristics of Staphylococcus aureus isolates from bovine mastitis.

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          Abstract

          Staphylococcus aureus is one of the major etiological agents of bovine mastitis, harboring a wide variety of staphylococcal superantigen (SAg) toxin genes. The SAg toxin genes are reported to be closely associated with the pathogenicity of the Staph. aureus causing the bovine mastitis. This study was conducted to investigate SAg toxin gene profiles and to assess the relationships among SAg toxin genes, genotypes of Staph. aureus, and their pathogenic properties. A total of 327 quarter milk samples were collected from bovine mastitis cases for isolation and identification of pathogens. In total, 35 isolates were identified as Staph. aureus, and the prevalence of Staph. aureus in milk samples was 13.6% (35/256). Polymerase chain reaction (PCR) and randomly amplified polymorphic DNA (RAPD) assays were used to detect the SAg toxin genes and to genotype Staph. aureus strains isolated from milk samples of bovine mastitis in 10 dairy herds located in Ningxia, China, respectively. The results showed that among the Staph. aureus isolates (n = 35), 71.4% (n = 25) of isolates carried at least one SAg toxin gene. In total, 18 SAg genes and 21 different gene combination patterns were detected among these isolates. The most common SAg genes in Staph. aureus isolates were sei, sen, and seu (44.0% each), followed by seo, tst, and etB (28.0% each), etA (24.0%), sem and sep (16.0% each), seb, sec, sed, and sek (12.0% each), and sea and seh genes (8.0% each); the seg, sej, and ser genes were present in 4.0% of the isolates. Three gene combinations were found to be related to mobile genetic elements that carried 2 or more genes. The egc-cluster of the seg-sei-sem-sen-seo genes, located on the pathogenicity island Type I υSaβ, was detected in 16% of isolates. Interestingly, we observed 6 RAPD genotypes (I to VI) in Staph. aureus isolates, and 2 of these genotypes were strongly associated with the severity of bovine mastitis; there was a close relationship between the RAPD genotypes and SAg genes. Isolates of RAPD type III were more frequently associated with clinical and subclinical mastitis, whereas strains of type VI were mostly related to subclinical mastitis. In addition, SAg genes were related to severity of bovine mastitis. We conclude that an obvious relationship exists among RAPD genotypes, SAg toxin genes, and severity of bovine mastitis.

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          Author and article information

          Journal
          J. Dairy Sci.
          Journal of dairy science
          American Dairy Science Association
          1525-3198
          0022-0302
          Jun 2017
          : 100
          : 6
          Affiliations
          [1 ] Key Laboratory of the Ministry of Education for the Conservation and Utilization of Special Biological Resources of Western China, College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China.
          [2 ] College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
          [3 ] Animal Disease Control and Prevention Center of Shapotou District, Zhongwei, Ningxia 755000, China.
          [4 ] Agriculture and Animal Husbandry Bureau of Shizuishan, Shizuishan, Ningxia 753000, China.
          [5 ] Ningxia Center for Disease Control and Prevention, Yinchuan, Ningxia 750004, China.
          [6 ] College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. Electronic address: hanbo@cau.edu.cn.
          Article
          S0022-0302(17)30336-3
          10.3168/jds.2016-12405
          28434738

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