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      Prenatal diagnosis of parvovirus B19-induced hydrops fetalis by chemiluminescence in situ hybridization.

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          Abstract

          Parvovirus B19 can be transmitted transplacentally from the infected mother to the fetus during pregnancy, and hydrops fetalis, abortion, or stillbirth can result. In our study we explored the use of chemiluminescence in situ hybridization to detect B19 DNA on cord blood cells, amniotic fluid cells, and pleuric fluid cells from several cases of hydrops fetalis. B19 DNA was detected by using digoxigenin-labeled probes immunoenzymatically visualized with the chemiluminescent adamantil-1,2-dioxetane phenyl phosphate substrate for alkaline phosphatase. The luminescent signal emitted from the hybridized probes was detected, analyzed, and measured with a high-performance, low-light-level imaging luminograph connected to an optical microscope and to a personal computer for the quantification and localization of the chemiluminescent emission inside individual cells.

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          Author and article information

          Journal
          J. Clin. Microbiol.
          Journal of clinical microbiology
          0095-1137
          0095-1137
          Jul 1999
          : 37
          : 7
          Affiliations
          [1 ] Division of Microbiology, Department of Clinical and Experimental Medicine, University of Bologna, Bologna, Italy. musiani@med.unibo.it
          85149
          10364606

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