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      Aptamer based electrochemical sensors for emerging environmental pollutants

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          Abstract

          Environmental contaminants monitoring is one of the key issues in understanding and managing hazards to human health and ecosystems. In this context, aptamer based electrochemical sensors have achieved intense significance because of their capability to resolve a potentially large number of problems and challenges in environmental contamination. An aptasensor is a compact analytical device incorporating an aptamer (oligonulceotide) as the sensing element either integrated within or intimately associated with a physiochemical transducer surface. Nucleic acid is well known for the function of carrying and passing genetic information, however, it has found a key role in analytical monitoring during recent years. Aptamer based sensors represent a novelty in environmental analytical science and there are great expectations for their promising performance as alternative to conventional analytical tools. This review paper focuses on the recent advances in the development of aptamer based electrochemical sensors for environmental applications with special emphasis on emerging pollutants.

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          Most cited references90

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          Nanomaterials based on DNA.

          The combination of synthetic stable branched DNA and sticky-ended cohesion has led to the development of structural DNA nanotechnology over the past 30 years. The basis of this enterprise is that it is possible to construct novel DNA-based materials by combining these features in a self-assembly protocol. Thus, simple branched molecules lead directly to the construction of polyhedrons, whose edges consist of double helical DNA and whose vertices correspond to the branch points. Stiffer branched motifs can be used to produce self-assembled two-dimensional and three-dimensional periodic lattices of DNA (crystals). DNA has also been used to make a variety of nanomechanical devices, including molecules that change their shapes and molecules that can walk along a DNA sidewalk. Devices have been incorporated into two-dimensional DNA arrangements; sequence-dependent devices are driven by increases in nucleotide pairing at each step in their machine cycles.
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            Aptamers from cell-based selection for bioanalytical applications.

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              High-resolution molecular discrimination by RNA.

              Species of RNA that bind with high affinity and specificity to the bronchodilator theophylline were identified by selection from an oligonucleotide library. One RNA molecule binds to theophylline with a dissociation constant Kd of 0.1 microM. This binding affinity is 10,000-fold greater than the RNA molecule's affinity for caffeine, which differs from theophylline only by a methyl group at nitrogen atom N-7. Analysis by nuclear magnetic resonance indicates that this RNA molecule undergoes a significant change in its conformation or dynamics upon theophylline binding. Binding studies of compounds chemically related to theophylline have revealed structural features required for the observed binding specificity. These results demonstrate the ability of RNA molecules to exhibit an extremely high degree of ligand recognition and discrimination.

                Author and article information

                Contributors
                Journal
                Front Chem
                Front Chem
                Front. Chem.
                Frontiers in Chemistry
                Frontiers Media S.A.
                2296-2646
                26 June 2014
                2014
                : 2
                : 41
                Affiliations
                [1] 1BIOMEM, Université de Perpignan Perpignan, France
                [2] 2Interdisciplinary Research Centre in Biomedical Materials, COMSATS Institute of Information Technology Lahore, Pakistan
                Author notes

                Edited by: Margarita Stilianova Stoytcheva, Universidad Autonoma de Baja California, Mexico

                Reviewed by: Bing Yu, Qingdao University, China; Sujuan Ye, Qingdao University of Science and Technology, China

                *Correspondence: Jean L. Marty, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan Cedex 66860, France e-mail: jlmarty@ 123456univ-perp.fr

                This article was submitted to Analytical Chemistry, a section of the journal Frontiers in Chemistry.

                Article
                10.3389/fchem.2014.00041
                4071757
                25019067
                10af722c-586a-4742-9f05-4aceef12aee2
                Copyright © 2014 Hayat and Marty.

                This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

                History
                : 16 April 2014
                : 09 June 2014
                Page count
                Figures: 4, Tables: 1, Equations: 0, References: 91, Pages: 9, Words: 7423
                Categories
                Chemistry
                Review Article

                electrochemical sensor,aptamer,emerging pollutants,environmental applications,analytical monitoring

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