7
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Failure of filipin to detect cholesterol-rich domains in smooth muscle plasma membrane.

      ,
      Nature

      Read this article at

      ScienceOpenPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Using the cholesterol probe filipin and freeze-fracture electron microscopy, Montesano reported an apparent difference in cholesterol content between contiguous zones of the smooth muscle plasma membrane. In this membrane, microinvaginations termed caveolae are organized in band-like domains, which were highly sensitive to filipin (and therefore cholesterol-rich) compared with the surrounding non-caveolar inter-band regions (assumed to be cholesterol-poor). Using another cholesterol probe, the saponin tomatin, applied alone and after filipin treatment, we now present evidence that both the band and inter-band domains of the smooth muscle plasma membrane are cholesterol-rich. This leads to the conclusion that the lack of inter-band response to filipin is a false-negative cytochemical result.

          Related collections

          Author and article information

          Journal
          Nature
          Nature
          0028-0836
          0028-0836
          : 303
          : 5918
          Article
          6855909
          10f7518f-8eb1-4b8a-9f00-62ffd4fbb928
          History

          Comments

          Comment on this article