+1 Recommend
1 collections
      • Record: found
      • Abstract: found
      • Article: found

      Progesterone and Estrogen Receptors in GH3 Cells

      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.


          GH3 cells are shown to contain cytosolic progesterone receptor in a mean concentration of 270 fmol/mg protein with a dissociation constant (K<sub>D</sub>) for promegestone of 3 × 10<sup>-9</sup> M (4°C). Estrogen receptor (K<sub>D</sub> = 1.8 × 10<sup>-10</sup> M) is demonstrated in cytosol (121 fmol/mg protein) as well as in 0.4 M KCl extracts (89 fmol/mg protein) of crude nuclear fractions. No progesterone receptor was detectable in the nuclear fraction. Both receptors are characterized by isoelectric focussing and by competition experiments. Addition of 10<sup>-8</sup> M estradiol to the growth medium increases progesterone receptor levels up to five, suggesting that the progesterone receptor is under estrogenic control.

          Related collections

          Author and article information

          Horm Res Paediatr
          Hormone Research in Paediatrics
          S. Karger AG
          25 November 2008
          : 12
          : 6
          : 324-332
          Laboratory for Experimental Endocrinology, Department of Gynecology, University Clinic, Basel
          179138 Horm Res 1980;12:324–332
          © 1980 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 9
          Original Paper


          Comment on this article