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      Coordinate transcriptional and translational repression of p53 by TGF-β1 impairs the stress response.

      Molecular Cell

      drug effects, Apoptosis, genetics, Base Sequence, Blotting, Western, Breast Neoplasms, metabolism, pathology, Cell Cycle, Cell Line, Cell Survival, Cells, Cultured, E2F4 Transcription Factor, Epithelial Cells, Gene Expression Regulation, Humans, Immunohistochemistry, Mammary Glands, Human, cytology, Molecular Sequence Data, Promoter Regions, Genetic, Protein Binding, RNA Interference, Reverse Transcriptase Polymerase Chain Reaction, Ribosomal Proteins, Signal Transduction, Smad Proteins, Stress, Physiological, Transforming Growth Factor beta1, pharmacology, Tumor Suppressor Protein p53

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          Abstract

          Cellular stress results in profound changes in RNA and protein synthesis. How cells integrate this intrinsic, p53-centered program with extracellular signals is largely unknown. We demonstrate that TGF-β1 signaling interferes with the stress response through coordinate transcriptional and translational repression of p53 levels, which reduces p53-activated transcription, and apoptosis in precancerous cells. Mechanistically, E2F-4 binds constitutively to the TP53 gene and induces transcription. TGF-β1-activated Smads are recruited to a composite Smad/E2F-4 element by an E2F-4/p107 complex that switches to a Smad corepressor, which represses TP53 transcription. TGF-β1 also causes dissociation of ribosomal protein RPL26 and elongation factor eEF1A from p53 mRNA, thereby reducing p53 mRNA association with polyribosomes and p53 translation. TGF-β1 signaling is dominant over stress-induced transcription and translation of p53 and prevents stress-imposed downregulation of Smad proteins. Thus, crosstalk between the TGF-β and p53 pathways defines a major node of regulation in the cellular stress response, enhancing drug resistance. Copyright © 2013 Elsevier Inc. All rights reserved.

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          Author and article information

          Journal
          23706820
          3735454
          10.1016/j.molcel.2013.04.029

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