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      Adjusting batch effects in microarray expression data using empirical Bayes methods.

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          Abstract

          Non-biological experimental variation or "batch effects" are commonly observed across multiple batches of microarray experiments, often rendering the task of combining data from these batches difficult. The ability to combine microarray data sets is advantageous to researchers to increase statistical power to detect biological phenomena from studies where logistical considerations restrict sample size or in studies that require the sequential hybridization of arrays. In general, it is inappropriate to combine data sets without adjusting for batch effects. Methods have been proposed to filter batch effects from data, but these are often complicated and require large batch sizes ( > 25) to implement. Because the majority of microarray studies are conducted using much smaller sample sizes, existing methods are not sufficient. We propose parametric and non-parametric empirical Bayes frameworks for adjusting data for batch effects that is robust to outliers in small sample sizes and performs comparable to existing methods for large samples. We illustrate our methods using two example data sets and show that our methods are justifiable, easy to apply, and useful in practice. Software for our method is freely available at: http://biosun1.harvard.edu/complab/batch/.

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          Author and article information

          Journal
          Biostatistics
          Biostatistics (Oxford, England)
          Oxford University Press (OUP)
          1465-4644
          1465-4644
          Jan 2007
          : 8
          : 1
          Affiliations
          [1 ] Department of Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, MA, USA.
          Article
          kxj037
          10.1093/biostatistics/kxj037
          16632515
          11ab4890-8726-43f2-b7d7-25308c50a127
          History

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