Fistulinella cinereoalba sp. nov., Austroboletus rostrupii, previously known from southeastern Asia, and Austroboletus festivus from Brazilian Amazonia are described for the first time from the Guiana Shield. These boletes were collected from tropical forests dominated by ectomycorrhizal Dicymbe spp. (Fabaceae subfam. Caesalpinioideae) in the Pakaraima Mountains of western Guyana.