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      In situ Analysis of Coronary Terminal Arteriole Diameter Responses: Technical Report of a New Experimental Model

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          Abstract

          Introduction: To date, investigation of coronary arteriole vasomotor activity has been limited to arterioles >30– 40 µm. Here, we introduce a new experimental model to allow for in situ microscopy of terminal coronary arterioles. Methods: Rat hearts were perfused in a closed loop system (priming volume 20 ml) which was placed on a computer-controlled microscope stage. FITC-dextran and tetrodotoxin (TTX, 50 µ M) were added. Tilting of the microscope by 90° allowed for visual access to the ventricular surface. Arterioles were identified by the flow direction of fluorescent beads (1 µm). Images were recorded on video tape, and arteriole diameters were measured offline. Stability of the preparation and maintenance of coronary flow reserve were analyzed. Responses of coronary flow and arteriole diameters to the vasodilators papaverine and Na-nitroprusside were recorded. Results: In TTX-arrested control hearts coronary flow and terminal arteriole diameters were stable for 2 h. Administration of papaverine and Na-nitroprusside increased coronary flow from 6.4 ± 0.7 to 13.3 ± 1.3 ml/min, decreasing coronary resistance by 52 ± 3%. Terminal coronary arteriole diameters increased from 12.0 ± 0.9 to 13.6 ± 1.0 µm, decreasing hindrance of this vessel segment by 45 ± 11%. Conclusion: Preservation of coronary terminal arteriolar tone and adequate responsiveness to vasodilators in the TTX-arrested isolated heart were demonstrated. Thus, this model may serve to complement our understanding of coronary microvascular control mechanisms by extending observations to the terminal arteriolar bed.

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          Efficacy of myocardial initial reperfusion with 2,3 butanedione monoxime after cardioplegic arrest is time-dependent

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            Time Course of Perfusion-Induced Myocardial Edema Resolution in Rats

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              A new microscope system for the continuous observation of the coronary microcirculation in the beating canine left ventricle

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                Author and article information

                Journal
                JVR
                J Vasc Res
                10.1159/issn.1018-1172
                Journal of Vascular Research
                S. Karger AG
                1018-1172
                1423-0135
                2003
                October 2003
                03 October 2003
                : 40
                : 5
                : 442-448
                Affiliations
                aDepartment of Physiology, University Hospital Benjamin Franklin, Freie Universität Berlin and bInstitute of Anesthesiology, Deutsches Herzzentrum Berlin, Berlin, Germany
                Article
                73909 J Vasc Res 2003;40:442–448
                10.1159/000073909
                14530601
                © 2003 S. Karger AG, Basel

                Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

                Page count
                Figures: 4, Tables: 2, References: 19, Pages: 7
                Categories
                Research Paper

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