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      Pharmacokinetic study of dendrobine in rat plasma by ultra-performance liquid chromatography tandem mass spectrometry.

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          Abstract

          Dendrobine, considered as the major active alkaloid compound, has been used for the quality control and discrimination of Dendrobium which is documented in the Chinese Pharmacopoeia. In this work, a sensitive and simple ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for determination of dendrobine in rat plasma is developed. After addition of caulophyline as an internal standard (IS), protein precipitation by acetonitrile-methanol (9:1, v/v) was used to prepare samples. Chromatographic separation was achieved on a UPLC BEH C18 (2.1 ×100 mm, 1.7 µm) column with acetonitrile and 0.1% formic acid as the mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; multiple reaction monitoring mode was used for quantification using target fragment ions m/z 264.2 → 70.0 for dendrobine and m/z 205.1 → 58.0 for IS. Calibration plots were linear throughout the range 2-1000 ng/mL for dendrobine in rat plasma. The RSDs of intra-day and inter-day precision were both <13%. The accuracy of the method was between 95.4 and 103.9%. The method was successfully applied to pharmacokinetic study of dendrobine after intravenous administration. Copyright © 2015 John Wiley & Sons, Ltd.

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          Author and article information

          Journal
          Biomed. Chromatogr.
          Biomedical chromatography : BMC
          Wiley
          1099-0801
          0269-3879
          Jul 2016
          : 30
          : 7
          Affiliations
          [1 ] The Laboratory of Clinical Pharmacy, The People's Hospital of Lishui, Wenzhou Medical University, Lishui, 323000, China.
          [2 ] Department of Anesthesiology, Critical Care and Pain Medicine, the Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, 325000, China.
          [3 ] The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325035, China.
          [4 ] Analytical and Testing Center, Wenzhou Medical University, Wenzhou, 325035, China.
          [5 ] Laboratory Animal Center, Wenzhou Medical University, Wenzhou, 325035, China.
          Article
          10.1002/bmc.3641
          26525040

          rat plasma, pharmacokinetics, UPLC-MS/MS, dendrobine

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