Timed pulses of WNT and FGF signaling convert human and mouse pluripotent stem cells into neuromesodermal progenitors that can be directed to differentiate into spinal cord and paraxial mesoderm cells
Cells of the spinal cord and somites arise from shared, dual-fated precursors, located towards the posterior of the elongating embryo. Here we show that these neuromesodermal progenitors (NMPs) can readily be generated in vitro from mouse and human pluripotent stem cells by activating Wnt and Fgf signalling, timed to emulate in vivo development. Similar to NMPs in vivo, these cells co-express the neural factor Sox2 and the mesodermal factor Brachyury and differentiate into neural and paraxial mesoderm in vitro and in vivo. The neural cells produced by NMPs have spinal cord but not anterior neural identity and can differentiate into spinal cord motor neurons. This is consistent with the shared origin of spinal cord and somites and the distinct ontogeny of the anterior and posterior nervous system. Systematic analysis of the transcriptome during differentiation identifies the molecular correlates of each of the cell identities and the routes by which they are obtained. Moreover, we take advantage of the system to provide evidence that Brachyury represses neural differentiation and that signals from mesoderm are not necessary to induce the posterior identity of spinal cord cells. This indicates that the mesoderm inducing and posteriorising functions of Wnt signalling represent two molecularly separate activities. Together the data illustrate how reverse engineering normal developmental mechanisms allows the differentiation of specific cell types in vitro and the analysis of previous difficult to access aspects of embryo development.
Stem cells are providing insight into embryo development and offering new approaches to clinical and therapeutic research. In part this progress arises from “directed differentiation” – artificially controlling the types of cells produced from stem cells. Here we describe the directed differentiation of mouse and human pluripotent stem cells into cells of the spinal cord and paraxial mesoderm (the tissue that generates muscle and bone that is normally found adjacent to the spinal cord). During embryo development, spinal cord and paraxial mesoderm arise from a shared group of precursors known as neuromesodermal progenitors (NMPs). We show that signals to which NMPs are exposed in embryos can be used to generate NMPs from pluripotent stem cells in a dish. We define conditions for the conversion of these NMPs into either spinal cord or mesoderm cells. Using these conditions, we provide evidence that the decision between spinal cord and mesoderm involves a gene, Brachyury, that promotes mesoderm production by inhibiting spinal cord generation. Together the data illustrate how mimicking normal embryonic development allows the generation of specific cell types from stem cells and that this can be used to analyse cells that are otherwise difficult to study.