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      Surveillance of SARS-CoV-2 in the environment and animal samples of the Huanan Seafood Market

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          Abstract

          Emerging in December 2019, coronavirus disease 2019 (COVID-19) eventually became a pandemic and has posed a tremendous threat to global public health. However, the origins of SARS-CoV-2, the causative agent of COVID-19, remain to be determined. It has reported that a certain number of the early case clusters had a contact history with Huanan Seafood Market. Therefore, surveillance of SARS-CoV-2 within the market is of vital importance. Herein, we presented the SARS-CoV-2 detection results of 1380 samples collected from the environment and the animals within the market in early 2020. By SARS-CoV-2-specific RT-qPCR, 73 environmental samples tested positive for SARS-CoV-2 and three live viruses were successfully isolated. The viruses from the market shared nucleotide identity of 99.980% to 99.993% with the human isolate HCoV/Wuhan/IVDC-HB-01. In contrast, no virus was detected in the animal swabs covering 18 species of animals in the market. The SARS-COV-2 nucleic acids in the positive environmental samples showed significant correlation of abundance of Homo sapiens with SARS-CoV-2. In summary, this study provided convincing evidence of the prevalence of SARS-CoV-2 in the Huanan Seafood Market during the early stage of COVID-19 outbreak.

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          Contributors
          Journal
          Research Square
          February 25 2022
          Affiliations
          [1 ]Chinese Academy of Sciences
          [2 ]National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention
          [3 ]MHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, China CDC
          [4 ]NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Contol and Prevention
          [5 ]Hubei Provincial Center for Disease Control and Prevention
          [6 ]Key Laboratory of Etiology and Epidemiology of Emerging Infectious Disease in Universities of Shandong, Shandong First Medical University, and Shandong Academy of Medical Science
          [7 ]Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine, Ruijin Hospital Affiliated to Shanghai Jiao Tong School of Medicine
          [8 ]Public Health Agency of Canada
          [9 ]National Institute For Viral Disease Control and Prevention, Chinese Center For Disease Control and Prevention
          [10 ]Institute of Microbiology, Chinese Academy of Sciences
          [11 ]Hubei Center for Disease Control and Prevention
          [12 ]China CDC
          Article
          10.21203/rs.3.rs-1370392/v1
          12571438-dee5-49da-9241-fd88fee07e8c
          © 2022

          https://creativecommons.org/licenses/by/4.0/

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