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      Quantification of inter-erythrocyte forces with ultra-high frequency (410 MHz) single beam acoustic tweezer

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          Abstract

          Efforts on quantitative measurements of the interactive forces of red blood cells (RBC) have been pursued for many years in hopes of a better understanding of hemodynamics and blood rheology. In this paper, we report an approach based on an ultra-high frequency (410 MHz) single beam acoustic tweezer (SBAT) for quantitative measurements of inter-RBC forces at a single cell level. The trapping forces produced by this ultra-high frequency (UHF) SBAT can be quantitatively estimated with a micropipette. Since the focal beam diameter of the 410 MHz ultrasonic transducer used in this SBAT was only 6.5 micrometer (μm), which was smaller than that of a RBC (~7.5 μm), it was made possible to directly apply the beam to a single RBC and measure inter-RBC forces against the pre-calibrated acoustic trapping forces as another example of potential cellular applications of the SBAT. The magnitude of these forces was found to be 391.0 ± 86.4 pN. Finally, it is worth noting that unlike several other methods, this method does not require the measuring device to be in contact with the cells.

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          Author and article information

          Journal
          0361512
          561
          Ann Biomed Eng
          Ann Biomed Eng
          Annals of biomedical engineering
          0090-6964
          1573-9686
          14 June 2017
          30 May 2017
          September 2017
          01 September 2018
          : 45
          : 9
          : 2174-2183
          Affiliations
          [1 ]NIH Resource Center for Medical Ultrasonic Transducer Technology and Department of Biomedical Engineering, University of Southern California, Los Angeles, California, 90089, USA
          Author notes
          Corresponding author: Hae Gyun Lim 1, NIH Resource Center for Medical Ultrasonic Transducer Technology and Department of Biomedical Engineering, University of Southern California, 1042 Downey way, University Park, DRB 131, Los Angeles, CA, 90089, Phone: +1-310-866-7573, Fax: +1-213-821-3897
          Article
          PMC5709217 PMC5709217 5709217 nihpa880768
          10.1007/s10439-017-1863-z
          5709217
          28560553
          12f2c6c4-d030-42ba-86f3-3c238ab50d72
          History
          Categories
          Article

          Single beam acoustic tweezer,Ultra high frequency ultrasonic transducer,Acoustic trapping force,Red blood cell,Micropipette aspiration technique

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