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Metabolic and hematologic consequences of colectomy associated to hepatectomy in rats Translated title: Consequências metabólicas e hematológicas da colectomia associada à hepatectomia em ratos

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      Abstract

      PURPOSE: To investigate the influence of partial colectomy associated with hepatectomy on the biodistribution of the 99mTc-phytate, on metabolic parameters, as well as labeling and morphology of red blood cells. METHODS: Wistar rats were distributed into three groups (each with six), nominated as colectomy, colectomy+hepatectomy and sham. In the 30th postoperative day all rats were injected with 99mTc-phytate 0.1mL i.v. (radioactivity 0.66 MBq). After 15 minutes, liver sample was harvested and weighed. Percentage radioactivity per gram of tissue (%ATI/g) was determined using an automatic gamma-counter. Serum AST, ALT, alkaline phosphatase and red blood cells labeling were determined. RESULTS: The liver %ATI/g and red blood cells labeling were lower in colectomy and colectomy+hepatectomy rats than in sham rats (p <0.05), and no difference was detected comparing the colectomy and colectomy+hepatectomy groups. Red blood cells morphology did not differ among groups. Serum levels of AST, ALT and alkaline fosfatase were significantly higher in colectomy+hepatectomy than in colectomy rats (p<0.001). CONCLUSION: Hepatectomy associated with colectomy lowered the uptake of radiopharmaceutical in liver and in red blood cells in rats, coinciding with changes in liver enzymatic activity.

      Translated abstract

      OBJETIVO: Investigar a influência da colectomia associada à hepatectomia parcial, na biodistribuição do fitato-99mTcO4, na marcação e morfologia de hemácias e em parâmetros metabólicos. MÉTODOS: Ratos Wistar foram distribuídos em três grupos (seis animais cada), denominados: colectomia, colectomia+hepatectomia e sham. No 30º dia pós-operatório, em todos eles foi feita injeção de 0,1 mL i.v. de fitato-99mTcO4 (radioatividade 0,66 MBq). Após 15 minutos, uma amostra de fígado foi colhida e pesada. O percentual de radioatividade por grama de tecido (%ATI/g) foi determinado no fígado e hemácias usando-se um contador gama automático. Dosagem sérica de AST, ALT, fosfatase alcalina, morfologia e marcação de hemácias com pertecnetato foram determinadas. RESULTADOS: O %ATI/g no fígado e nas hemácias foi menor nos animais dos grupos colectomia e colectomia+hepatectomia do que no grupo sham (p<0,05; teste de Tukey). Nenhuma diferença foi detectada comparando os grupos colectomia e colectomia+hepatectomia. A morfologia das hemácias não diferiu entre os três grupos. Os níveis séricos de AST, ALT e fosfatase alcalina foram significativamente maiores no grupo colectomia+hepatectomia do que no grupo colectomia (p<0,001). CONCLUSÃO: A colectomia associada a hepatectomia contribuiu para reduzir a captação de radiofármaco no fígado e hemácias de ratos, coincidindo com alterações na atividade enzimática do fígado.

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      Most cited references 19

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      Recent advances in 99mTc radiopharmaceuticals.

       Yasushi Arano (2002)
      99mTc radiopharmaceuticals play an important role in widespread applications of nuclear medicine. When 99mTc radiopharmaceuticals first came into use, major efforts were directed toward the development of 99mTc radiopharmaceuticals for bone imaging and for the excretory functions of the liver and kidneys. In the past 20 years, a significant advance has been made in technetium chemistry, which provided 99mTc radiopharmaceuticals for assessment of regional cerebral and myocardial blood flow. Recent efforts have been directed toward the design of 99mTc-labeled compounds for estimating receptor or transporter functions. A number of bifunctional chelating agents that provide 99mTc labeled proteins and peptides of high in vivo stability with high radiochemical yields have also been developed. More recently, organometallic technetium and rhenium compounds have been introduced as another class of 99mTc radiopharmaceutical design. In this manuscript, recent progress in 99mTc radiopharmaceuticals is reviewed with the major emphasis laid on key innovations in this field to provide the 99mTc radiopharmaceuticals available today.
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        The role of nuclear medicine in the detection of acute gastrointestinal bleeding.

         D Howarth (2006)
        The clinical consequences of lower gastrointestinal bleeding range from trivial to life-threatening. Nuclear medicine imaging techniques allow identification of those patients who are actively bleeding. The demonstration of active bleeding not only provides important prognostic information but also allows for a coordinated imaging approach using angiography and aids localization of the bleeding site. (99m)Tc-labeled erythrocytes and (99m)Tc sulfur colloid are 2 commonly used techniques to detect active bleeding. Each has its respective advantages and disadvantages, but the medical literature indicates that both tests are useful. More prolonged or delayed imaging is possible using (99m)Tc-labeled erythrocytes but care is required to prevent misinterpretation of the bleeding location because of a higher likelihood of radiotracer movement through the bowel away from the bleeding site. These forms of scintigraphy may be helpful in risk-stratifying patients and planning radiological and surgical interventions. Careful selection of patients to include those who have a high likelihood of active bleeding greatly increases the clinical utility of these tests. In addition, (99m)Tc pertechnetate imaging may be diagnostic of ectopic gastric mucosa in a Meckel's diverticulum as a potential source of bleeding. Patients also should be carefully selected for this test, based on age and exclusion of other causes of bleeding.
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          Biodistribution of technetium-99m pertechnetate after total colectomy in rats.

          This study evaluated the effects of total colectomy on the biodistribution of technetium-99m pertechnetate ((99m)TcO(4)(-)) on the 28th postoperative day in rats. Samples of several organs were harvested for counting the percent of injected radioactivity/g of tissue (%ATI/g). The %ATI/g in colectomy rats was higher in the stomach and ileum than in sham and controls (p<0.05). Increase in mucosa and muscularis size of ileum was observed. Colectomy was associated with lower biodistribution in bladder and thyroid, T3, and T4, than in controls. Copyright 2010 Elsevier Ltd. All rights reserved.
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            Author and article information

            Affiliations
            [1 ] Universidade Federal do Rio Grande do Norte Brazil
            [2 ] Universidade Federal do Rio Grande do Norte Brazil
            [3 ] Universidade Federal do Rio Grande do Norte Brazil
            [4 ] Universidade Potiguar Brazil
            [5 ] Universidade Federal do Rio Grande do Norte Brazil
            Contributors
            Role: ND
            Role: ND
            Role: ND
            Role: ND
            Role: ND
            Journal
            acb
            Acta Cirurgica Brasileira
            Acta Cir. Bras.
            Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (São Paulo )
            1678-2674
            December 2011
            : 26
            : 6
            : 503-507
            S0102-86502011000600016
            10.1590/S0102-86502011000600016

            http://creativecommons.org/licenses/by/4.0/

            Product
            Product Information: SciELO Brazil
            Categories
            SURGERY

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