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      Effect of Cell Density on Hormonal Secretion from Human Pituitary Adenomas in vitro

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          Cell density effects were investigated on tumorous hormonal secretion from 10 pituitary adenomas: 3 somatotrophinomas secreting GH and PRL; 7 gonadotrophinomas, 3 co-secreted both FSH and LH, all 7 secreted LH. Enzymatically dispersed tissue was plated out in 24-well plates at 5 ×10<sup>5</sup>, 10<sup>5</sup>, 5 ×10<sup>4</sup> and 10<sup>4</sup> cells/well in serum-free media. Media were collected weekly for 2 weeks. Results: In 3 of 3 somatotrophinomas, GH and PRL secretion was higher (p < 0.05) at both week 1 and 2 from 10<sup>4</sup> cells/well, but similar at other cell densities. In all 3 gonadotrophinomas, the FSH secretory rate was highest at 5 ×10<sup>5</sup> cells/well which fell as cell density decreased. Conversely, in 7 of 7 gonadotrophinomas the LH secretory rate was highest at 10<sup>4</sup> cells/well (p < 0.01) which fell as cell density increased. Conclusion: These data suggest that paracrine factors may modulate tumorous GH, PRL, FSH and LH secretion, and show that FSH and LH secretion vary inversely as cell density increases.

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          Most cited references 6

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          New perspectives in the function of pituitary folliculo-stellate cells.

          Classical morphological studies of the folliculo-stellate (FS) cells of the anterior pituitary have suggested that these cells play roles as supporting cells, in metabolism and in macromolecular transport. Over the last 10 years the details of their activity in both trophic and catabolic processes has been clarified, and recent work has demonstrated several transport systems in these cells. Various novel peptides with growth factor or cytokine activity have been identified in FS cells and/or FS cell conditioned media. These recent functional experiments confirm and extend previous morphological and experimental studies, and in addition open new perspectives on the physiological roles of FS cells.
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            Bioactive peptides in anterior pituitary cells

             H. Houben,  C Denef (1994)
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              Anterior pituitary cells: getting to know their neighbors


                Author and article information

                Horm Res Paediatr
                Hormone Research in Paediatrics
                S. Karger AG
                May 1998
                03 April 1998
                : 49
                : 5
                : 203-209
                a Department of Medicine, University of Hull, UK; b Department of Chemical Pathology, University of Liverpool, UK; c Neurosurgical Department, Walton Hospital, Liverpool, UK, and d Zürich, Switzerland
                23172 Horm Res 1998;49:203–209
                © 1998 S. Karger AG, Basel

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                Page count
                Figures: 4, References: 21, Pages: 7
                Original Paper


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