Experimental corneal calcification has been produced in rabbits by a combination of corneal injury (perfusion of the anterior chamber with a solution of kalium permanganate) and dihydrotachysterol (DHT) treatment. Three groups of rabbits were compared: a group of animals pretreated with DHT and with perfusion of the anterior chamber of the left eye, a group of animals only injured, and a group of normal rabbits. Hydration of the corneas and penetration of intravenously injected <sup>45</sup>Ca into the aqueous, central and peripheral parts of corneas, were followed. The highest activity of aqueous and corneas of both eyes was found in rabbits treated with DHT. No significant difference between the radioactivity of the central and peripheral parts of the cornea was observed. Hydration of corneas in this group seemed to be less pronounced in comparison with the group of rabbits without DHT treatment.