13 July 2015
It has been suggested that oxygen (O 2) diffusion could be favored in water enriched in O 2 by a new electrolytic process because of O 2 trapping in water superstructures (clathrates), which could reduce the local pressure/content relationships for O 2 and facilitate O 2 diffusion along PO 2 gradients.
Mitochondrial respiration was compared in situ in saponin-skinned fibers isolated from the soleus muscles of Wistar rats, in solution enriched in O 2 by injection or the electrolytic process 1) at an O 2 concentration decreasing from 240 µmol/L to 10 µmol/L (132 mmHg to 5 mmHg), with glutamate–malate or N, N, N′, N′-tetramethyl- p-phenylenediamine dihydrochloride (TMPD)–ascorbate (with antimycin A) as substrates; and 2) at increasing adenosine diphosphate (ADP) concentration with glutamate–malate as substrate.
As expected, maximal respiration decreased with O 2 concentration and, when compared to glutamate–malate, the apparent Km O 2 of mitochondria for O 2 was significantly lower with TMPD–ascorbate with both waters. However, when compared to the water enriched in O 2 by injection, the Km O 2 was significantly lower with both electron donors in water enriched in O 2 by electrolysis. This was not associated with any increase in the sensitivity of mitochondria to ADP; no significant difference was observed for the Km ADP between the two waters.