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      A Multivariate‐Gated DNA Nanodevice for Spatioselective Imaging of Pro‐metastatic Targets in Extracellular Microenvironment

      1 , 2 , 2 , 1 , 1 , 2
      Angewandte Chemie
      Wiley

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          Abstract

          Probing pro‐metastatic biomarkers is of significant importance to evaluate the risk of tumor metastasis, but spatially selective imaging of such targets in extracellular microenvironment is particularly challenging. By introducing the bilinguality of PNA/peptide hybrid that can speak both peptide substrate and nucleobase‐pairing languages to combine with aptamer technology, we designed a smart DNA nanodevice programmed to respond sequentially to dual pro‐metastatic targets, MMP2/9 and ATP, in extracellular tumor microenvironment (TME). The DNA nanodevice is established based on the combination of an ATP‐responsive aptamer sensor and a MMP2/9‐hydrolyzable PNA/peptide copolymer with a cell membrane‐anchoring aptamer module. Taking 4T1 xenograft as a highly aggressive tumor model, the robustness of the DNA nanodevice in spatioselective imaging of MMP2/9 and ATP in TME is demonstrated. We envision that this design will enable the simultaneous visualization of multiple pro‐metastatic biomarkers, which allows to gain insights into their pathological roles in tumor metastasis.

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          Most cited references43

          • Record: found
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          Emerging Biological Principles of Metastasis.

          Metastases account for the great majority of cancer-associated deaths, yet this complex process remains the least understood aspect of cancer biology. As the body of research concerning metastasis continues to grow at a rapid rate, the biological programs that underlie the dissemination and metastatic outgrowth of cancer cells are beginning to come into view. In this review we summarize the cellular and molecular mechanisms involved in metastasis, with a focus on carcinomas where the most is known, and we highlight the general principles of metastasis that have begun to emerge.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Matrix metalloproteinases: regulators of the tumor microenvironment.

            Extracellular proteolysis mediates tissue homeostasis. In cancer, altered proteolysis leads to unregulated tumor growth, tissue remodeling, inflammation, tissue invasion, and metastasis. The matrix metalloproteinases (MMPs) represent the most prominent family of proteinases associated with tumorigenesis. Recent technological developments have markedly advanced our understanding of MMPs as modulators of the tumor microenvironment. In addition to their role in extracellular matrix turnover and cancer cell migration, MMPs regulate signaling pathways that control cell growth, inflammation, or angiogenesis and may even work in a nonproteolytic manner. These aspects of MMP function are reorienting our approaches to cancer therapy. Copyright 2010 Elsevier Inc. All rights reserved.
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              • Record: found
              • Abstract: found
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              A logic-gated nanorobot for targeted transport of molecular payloads.

              We describe an autonomous DNA nanorobot capable of transporting molecular payloads to cells, sensing cell surface inputs for conditional, triggered activation, and reconfiguring its structure for payload delivery. The device can be loaded with a variety of materials in a highly organized fashion and is controlled by an aptamer-encoded logic gate, enabling it to respond to a wide array of cues. We implemented several different logical AND gates and demonstrate their efficacy in selective regulation of nanorobot function. As a proof of principle, nanorobots loaded with combinations of antibody fragments were used in two different types of cell-signaling stimulation in tissue culture. Our prototype could inspire new designs with different selectivities and biologically active payloads for cell-targeting tasks.
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                Author and article information

                Contributors
                Journal
                Angewandte Chemie
                Angewandte Chemie
                Wiley
                0044-8249
                1521-3757
                January 21 2022
                December 02 2021
                January 21 2022
                : 134
                : 4
                Affiliations
                [1 ] Center for Biomedical Optics and Photonics (CBOP) & College of Physics and Optoelectronic Engineering China Key Laboratory of Optoelectronic Devices and Systems Shenzhen University Shenzhen 518060 China
                [2 ] CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Beijing 100190 China
                Article
                10.1002/ange.202111836
                135972df-7c78-40a6-ab08-f1964e045a1a
                © 2022

                http://onlinelibrary.wiley.com/termsAndConditions#vor

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