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Cultivation and characterization of limbal epithelial stem cells on contact lenses with a feeder layer: toward the treatment of limbal stem cell deficiency.
Ariel Gore
1 ,
Vered Horwitz ,
Hila Gutman ,
Liat Tveria ,
Liat Cohen ,
Orit Cohen-Jacob ,
Joseph Turetz ,
Patrick Michael McNutt ,
Shlomit Dachir ,
Tamar Kadar
Jan 2014
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Abstract
Limbal epithelial sheets are used to promote corneal surface reconstruction after the detection of limbal epithelial stem cell deficiency. The aim of this study was to evaluate a novel combination of limbal stem cells (LSCs) maintained on contact lenses (CLs) in the presence of a 3T3 feeder cell layer regarding preservation of stem cell phenotype and the potential use for future in vivo transplantation.