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      Colour-Based Binary Discrimination of Scarified Quercus robur Acorns under Varying Illumination

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          Abstract

          Efforts to predict the germination ability of acorns using their shape, length, diameter and density are reported in the literature. These methods, however, are not efficient enough. As such, a visual assessment of the viability of seeds based on the appearance of cross-sections of seeds following their scarification is used. This procedure is more robust but demands significant effort from experienced employees over a short period of time. In this article an automated method of acorn scarification and assessment has been announced. This type of automation requires the specific setup of a machine vision system and application of image processing algorithms for evaluation of sections of seeds in order to predict their viability. In the stage of the analysis of pathological changes, it is important to point out image features that enable efficient classification of seeds in respect of viability. The article shows the results of the binary separation of seeds into two fractions (healthy or spoiled) using average components of regular red-green-blue and perception-based hue-saturation-value colour space. Analysis of accuracy of discrimination was performed on sections of 400 scarified acorns acquired using two various setups: machine vision camera under uncontrolled varying illumination and commodity high-resolution camera under controlled illumination. The accuracy of automatic classification has been compared with predictions completed by experienced professionals. It has been shown that both automatic and manual methods reach an accuracy level of 84%, assuming that the images of the sections are properly normalised. The achieved recognition ratio was higher when referenced to predictions provided by professionals. Results of discrimination by means of Bayes classifier have been also presented as a reference.

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          Estimation of rice growth and nitrogen nutrition status using color digital camera image analysis

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            Robust Crop and Weed Segmentation under Uncontrolled Outdoor Illumination

            An image processing algorithm for detecting individual weeds was developed and evaluated. Weed detection processes included were normalized excessive green conversion, statistical threshold value estimation, adaptive image segmentation, median filter, morphological feature calculation and Artificial Neural Network (ANN). The developed algorithm was validated for its ability to identify and detect weeds and crop plants under uncontrolled outdoor illuminations. A machine vision implementing field robot captured field images under outdoor illuminations and the image processing algorithm automatically processed them without manual adjustment. The errors of the algorithm, when processing 666 field images, ranged from 2.1 to 2.9%. The ANN correctly detected 72.6% of crop plants from the identified plants, and considered the rest as weeds. However, the ANN identification rates for crop plants were improved up to 95.1% by addressing the error sources in the algorithm. The developed weed detection and image processing algorithm provides a novel method to identify plants against soil background under the uncontrolled outdoor illuminations, and to differentiate weeds from crop plants. Thus, the proposed new machine vision and processing algorithm may be useful for outdoor applications including plant specific direct applications (PSDA).
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              Seed-mass effects in four Mediterranean Quercus species (Fagaceae) growing in contrasting light environments.

              Three hypotheses have been proposed to explain the functional relationship between seed mass and seedling performance: the reserve effect (larger seeds retain a larger proportion of reserves after germinating), the metabolic effect (seedlings from larger seeds have slower relative growth rates), and the seedling-size effect (larger seeds produce larger seedlings). We tested these hypotheses by growing four Mediterranean Quercus species under different light conditions (3, 27, and 100% of available radiation). We found evidence for two of the three hypotheses, but none of the four species complied with all three hypotheses at the same time. The reserve effect was not found in any species, the metabolic effect was found in three species (Q. ilex, Q. pyrenaica, and Q. suber), and the seedling-size effect in all species. Light availability significantly affected the relationships between seed size and seedling traits. For Q. ilex and Q. canariensis, a seedling-size effect was found under all three light conditions, but only under the lowest light (3%) for Q. suber and Q. pyrenaica. In all species, the correlation between seed mass and seedling mass increased with a decrease in light, suggesting that seedlings growing in low light depend more upon their seed reserves. A causal model integrates the three hypotheses, suggesting that larger seeds generally produced larger seedlings.
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                Author and article information

                Contributors
                Role: Academic Editor
                Journal
                Sensors (Basel)
                Sensors (Basel)
                sensors
                Sensors (Basel, Switzerland)
                MDPI
                1424-8220
                18 August 2016
                August 2016
                : 16
                : 8
                : 1319
                Affiliations
                [1 ]Faculty of Electrical Engineering, Automatics, Computer Science and Biomedical Engineering, AGH University of Science and Technology, 30 Mickiewicza Ave., Kraków 30-059, Poland; rtad@ 123456ahg.edu.pl (R.T.); ap@ 123456agh.edu.pl (A.P.)
                [2 ]Department of Forest Work Mechanisation, Faculty of Forestry, University of Agriculture in Krakow, 46 29-listopada Ave., Kraków 31-425, Poland; rltylek@ 123456cyf-kr.edu.pl (P.T.); rlwalczy@ 123456cyf-kr.edu.pl (J.W.)
                Author notes
                [* ]Correspondence: mjk@ 123456agh.edu.pl ; Tel.: +48-12-617-38-73; Fax: +48-12-634-15-68
                Article
                sensors-16-01319
                10.3390/s16081319
                5017484
                27548173
                1395cc50-6076-4978-9b9d-fd113d7e57cb
                © 2016 by the authors; licensee MDPI, Basel, Switzerland.

                This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license ( http://creativecommons.org/licenses/by/4.0/).

                History
                : 01 June 2016
                : 03 August 2016
                Categories
                Article

                Biomedical engineering
                pedunculate oak,scarification,viability prediction,colour image processing,machine vision,image classification

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