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      Immunocytochemical localization of microtubule-associated protein 1 in rat cerebellum using monoclonal antibodies

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      The Journal of Cell Biology
      The Rockefeller University Press

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          Abstract

          Immunohistochemical staining with monoclonal antibodies showed that microtubule-associated protein 1 (MAP1) has a restricted cellular distribution in the rat cerebellum. Anti-MAP1 staining was found only in neurons, where it was much stronger in dendrites than in axons. There were striking variations in the apparent concentration of MAP1 in different classes of neurons. Purkinje cells were the most strongly labeled, while granule cell neurons gave a faint, threshold-level reaction with the antibody. The reaction of Golgi neurons was intermediate between these two extremes. Equivalent results were obtained using two different methods of tissue preparation. Thus MAP1 appears to be a neuron-specific protein that is highly concentrated in dendrites and occurs at markedly different levels in different types of neurons. These observations provide further indications of heterogeneity among brain microtubules.

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          Author and article information

          Journal
          J Cell Biol
          The Journal of Cell Biology
          The Rockefeller University Press
          0021-9525
          1540-8140
          1 February 1984
          : 98
          : 2
          : 777-781
          Article
          84111796
          2113089
          6363428
          13a7e955-8adc-4c7a-86d8-445e2eaa546f
          History
          Categories
          Articles

          Cell biology
          Cell biology

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