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      Computer-Based Video Analysis of Cerebral Arterial Geometry Using the Natural Fluorescence of the Arterial Wall and Contrast Enhancement Techniques


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          The walls of mammalian arteries fluoresce with ultraviolet excitation in the wavelength range of 350–400 nm. We are using this property of natural fluorescence in conjunction with episcopic fluorescence microscopy, frozen section techniques, selective contrast enhancement, and a semi-automatic computer-based video analysis system to obtain dimensional data and 3-D coordinate maps of cerebral bifurcation geometry. The computer-video analysis system, comprised of commercially available video components, a Cromemco Z2D Computer, and electronic components built in our lab has a resolution of ± 1/256 mm at full-screen magnification. Our technique is based upon measurement of the video image of the block face during frozen sectioning of cerebral bifurcations. This technique allows us to obtain axially symmetric coordinate maps of cerebral bifurcation geometry without the use of block markers. In addition to mapping lumenal and adventitial surfaces the system can be used to map atheromatous lesions and the outline of the internal elastic lamina. Our initial geometrical data suggest a linear increase of 50–60% in cross-sectional area in the apical region and widening of the apical region in the plane of the daughter branches.

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          Author and article information

          J Vasc Res
          Journal of Vascular Research
          S. Karger AG
          19 September 2008
          : 20
          : 4
          : 161-171
          Department of Biophysics, Health Sciences Centre, University of Western Ontario, London, Ont., Canada
          158470 Blood Vessels 1983;20:161–171
          © 1983 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          : 29 June 1982
          : 14 July 1982
          Page count
          Pages: 11
          Research Paper

          General medicine,Neurology,Cardiovascular Medicine,Internal medicine,Nephrology
          Image analysis,Geometry,Cerebral bifurcation,Human artery,Elastin,Fluorescence


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