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      Sites of replication of a porcine respiratory coronavirus related to transmissible gastroenteritis virus

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      Research in Veterinary Science
      Published by Elsevier Ltd.

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          Abstract

          A porcine respiratory coronavirus ( prcv) was inoculated by aerosol into nine hysterectomy-derived and colostrum-deprived pigs at the age of one week. They were killed at different times after inoculation and tissues were sampled for virus isolation and immunofluorescence. Results indicate that virus replicated to high titres in the respiratory tract. Replication mainly occurred in alveolar cells but also in epithelial cells of nasal mucosa, trachea, bronchi, bronchioli, in alveolar macrophages and in tonsils. After primary replication in the respiratory tract, viraemia occurred. Virus also reached the gastrointestinal tract after swallowing. Subsequently, PRCV was observed to replicate in the ileum. The infection spread within a few days from the ileum to the duodenum. Replication in the small intestine remained limited to a few cells located in or underneath the epithelial layer of villi and, or, crypts. The cell type could not be identified. Virus was isolated from mesenteric lymph nodes in all pigs, but immunofluorescence was not observed. Results show that small changes in molecular structure between transmissible gastroenteritis virus and prcv resulted in important changes in host cell tropism.

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          Author and article information

          Journal
          Res Vet Sci
          Res. Vet. Sci
          Research in Veterinary Science
          Published by Elsevier Ltd.
          0034-5288
          1532-2661
          23 August 2018
          March 1990
          23 August 2018
          : 48
          : 2
          : 165-169
          Affiliations
          [1]Laboratory of Veterinary Virology and Immunology, Casinoplein 24, B-9000 Ghent, Belgium
          Article
          S0034-5288(18)30984-6
          10.1016/S0034-5288(18)30984-6
          7130871
          2159175
          13df03d0-cac8-4f87-b0e1-221ee6c9c29d
          Copyright © 1990 Published by Elsevier Ltd.

          Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

          History
          : 8 May 1989
          : 19 June 1989
          Categories
          Article

          Veterinary medicine
          Veterinary medicine

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