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      Pterostilbene attenuates myocardial ischemia-reperfusion injury via the phosphatidylinositol 3′-kinase-protein kinase B signaling pathway

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          Abstract

          The current study aimed to evaluate the cardioprotective effects of pterostilbene (PTB) on myocardial ischemia-reperfusion (I/R) injury in rats and identify its possible underlying mechanisms of action. A rat I/R model was established by ligating the left anterior descending coronary artery for 30 min and releasing the ligature to induce reperfusion for 120 min. Serum creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH) levels were measured using CK-MB and LDH assay kits and myeloperoxidase (MPO) activity in the myocardium was evaluated using an MPO assay kit. Tumor necrosis factor-α, interleukin (IL)-6 and IL-8 levels were assayed using ELISA kits. Cardiomyocyte apoptosis was measured using terminal deoxynucleotidyl transferase dUTP nick end labeling staining. Levels of protein kinase B (Akt) and phosphorylated Akt (p-Akt) were measured using western blotting. The results demonstrated that treatment with PTB significantly reduced cardiomyocyte apoptosis, significantly increased Bcl-2 and p-Akt levels and decreased Bax expression in the hearts of rats subjected to I/R injury. However, the protective effects induced by PTB were attenuated by LY294002, which inhibits Akt activation. The results of the current study suggest that PTB treatment may reduce the I/R injury-induced apoptosis of cardiomyocytes, which is mediated by the phosphoinositide 3-kinase/Akt signaling pathway.

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          Author and article information

          Journal
          Exp Ther Med
          Exp Ther Med
          ETM
          Experimental and Therapeutic Medicine
          D.A. Spandidos
          1792-0981
          1792-1015
          December 2017
          02 October 2017
          02 October 2017
          : 14
          : 6
          : 5509-5514
          Affiliations
          [1 ]Department of Clinical Laboratory, Northwest Women and Children's Hospital, Xi'an, Shaanxi 710061, P.R. China
          [2 ]Department of Medical Genetics, Northwest Women and Children's Hospital, Xi'an, Shaanxi 710061, P.R. China
          Author notes
          Correspondence to: Dr Hui Jia, Department of Clinical Laboratory, Northwest Women and Children's Hospital, 1616 Yanxiang Road, Xi'an, Shaanxi 710061, P.R. China, E-mail: clinicallabnwc@ 123456sina.com
          [*]

          Contributed equally

          Article
          PMC5740594 PMC5740594 5740594 ETM-0-0-5246
          10.3892/etm.2017.5246
          5740594
          29285084
          1405f0af-d35c-4692-b49b-20e33484aed5
          Copyright © 2017, Spandidos Publications
          History
          : 27 October 2016
          : 23 June 2017
          Categories
          Articles

          myocardial ischemia-reperfusion injury,phosphoinositide 3-kinase/protein kinase B signaling,pterostilbene

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