We studied the species of amphibians from Serranía de Las Quinchas, in the mid Magdalena Valley region of Colombia, comparing the data from a recent fieldwork with museum records and literature. We present a list of 50 species of amphibians (Anura and Caudata). In our survey, we recorded 36 species, of which 6 represented new records. One of the most interesting record is that of the genus Oedipina, since this is the first time the genus is recorded for the Cordillera Oriental. The number of species is what would be expected in a humid tropical forest of the mid-Magdalena river valley, given the co-occurrence of amphibian faunas distributed in the sub-Andean, Caribbean, and Chocó biogeographic regions.