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      miRNA-146a attenuates inflammation in an in vitro spinal cord injury model via inhibition of TLR4 signaling

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          Abstract

          The present study evaluated the anti-inflammatory effect of microRNA (miR)-146a in a spinal cord injury (SCI) rat model and in vitro model, and explored possible underlying mechanisms of this effect. miR-146a expression was analyzed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 content was measured using ELISA kits. Inducible nitric oxide synthase (iNOS), prostaglandin E2 (PGE2), Toll-like receptor 4 (TLR4), myeloid differentiation primary response 88 (MyD88) and phosphorylated (p)-nuclear factor (NF)-κB were measured using western blotting. In the SCI rat model, miR-146a expression was downregulated. In the in vitro model, downregulation of miR-146a increased inflammation, enhanced iNOS and PGE2 protein expression and induced TLR4, MyD88 and NF-κB expression. Overexpression of miR-146a reduced inflammation, iNOS and PGE2 protein expression, and suppressed TLR4, MyD88 and NF-κB expression in the in vitro SCI model. The inhibition of TLR4 attenuated the proinflammatory effects of anti-miR-146a in the in vitro SCI model. The results indicate that miR-146a reduces inflammation in an SCI model through the TLR4-NF-κB signaling pathway. The present study demonstrated that miR-146a may be a promising therapeutic agent for SCI.

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          Author and article information

          Journal
          Exp Ther Med
          Exp Ther Med
          ETM
          Experimental and Therapeutic Medicine
          D.A. Spandidos
          1792-0981
          1792-1015
          October 2018
          22 August 2018
          22 August 2018
          : 16
          : 4
          : 3703-3709
          Affiliations
          [1 ]Department of Spine Surgery, Weifang Traditional Chinese Medicine Hospital, Weifang, Shandong 261041, P.R. China
          [2 ]School of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China
          [3 ]Department of Orthopedics, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong 250014, P.R. China
          [4 ]Department of Spine Surgery, Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong 510430, P.R. China
          Author notes
          Correspondence to: Dr Chunming Zhang, Department of Orthopedics, Shandong Provincial Qianfoshan Hospital, Shandong University, 16766 Jingshi Road, Jinan, Shandong 250014, P.R. China, E-mail: zhangsurgeon@ 123456qq.com
          Dr Kebing Chen, Department of Spine Surgery, Third Affiliated Hospital of Southern Medical University, 183 Zhongshan Avenue (West), Guangzhou, Guangdong 510430, P.R. China, E-mail: chenkebing.sysu@ 123456gmail.com
          [*]

          Contributed equally

          Article
          PMC6143872 PMC6143872 6143872 ETM-0-0-6645
          10.3892/etm.2018.6645
          6143872
          30233729
          Copyright © 2018, Spandidos Publications
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