9
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Resistencia in vitro a acaricidas tópicos de poblaciones de garrapatas Rhipicephalus (Boophilus) microplus provenientes de cuatro departamentos de Colombia Translated title: In vitro resistance to topical acaricides of the cattle tick Rhipicephalus (Boophilus) microplus from four regions of Colombia Translated title: Resistência in vitro aos acaricidas tópicos de populações de carrapatos Rhipicephalus (Boophilus) microplus provenientes de quatro departamentos da Colô mbia

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          El control de la garrapata Rhipicephalus (Boophilus) microplus en Colombia radica casi exclusivamente en el uso de acaricidas químicos. Sin embargo, R. (B.) microplus ha desarrollado resistencia a la mayoría de los productos actualmente disponibles comercialmente. El objetivo de este estudio fue valorar el grado de resistencia a concentraciones recomendadas de los principales acaricidas comerciales de uso tópico en Colombia. Se empleó la prueba de inmersión de adultas (teleoginas) recomendada por la FAO con garrapatas procedentes de siete predios localizados en cuatro departamentos colombianos. La eficacia de los productos se calculó con base en los parámetros reproductivos de fecundidad y fertilidad con grupos de garrapatas (n=40) expuestas a cada acaricida, comparándolos con el respectivo grupo control expuesto a agua destilada. En general, los productos de deltametrina (25 ppm) y amitraz (208 ppm) fueron los menos efectivos, con valores de eficacia de <20% y de entre 10-50%, respectivamente. Tampoco se observó un aumento de eficacia al doblar la concentración de ambos compuestos por separado. La combinación de clorpirifos (312 ppm) + cipermetrina (150 ppm) mostró gran variación entre fincas, con una eficacia de entre el 22-93%. A diferencia  del clorpirifos, el organofosforado etión (622 ppm) sí produjo un 99-100% de eficacia inhibiendo completamente la oviposición en cuatro de las siete fincas. En conclusión, la resistencia a deltametrina y amitraz es muy alta en siete poblaciones distantes del territorio colombiano; en cuanto al clorpirifos varía mucho, y éste no parece tener resistencia cruzada con el etión que fue el de mayor eficacia.

          Translated abstract

          In Colombia, the control of the cattle tick Rhipicephalus (Boophilus) microplus lies almost exclusively on the use of chemical acaricides. However, R. (B.) microplus has developed resistance to most of the commercially available products. The aim of this study was to assess the degree of resistance to the major topical acaricides used in Colombia. The adult immersion test was used as recommended by FAO with ticks from 7 farms originating in 4 distant Colombian regions. The efficacy of the products was calculated based on the reproductive parameters of fecundity and fertility with groups of ticks (n = 40) exposed to the recommended concentrations of each acaricide, and compared with the respective control group exposed to distilled water. In general, products containing deltamethrin (25 ppm) and amitraz (208 ppm) were less effective, with efficacy values of <20% and 10-50%, respectively. Doubling the concentration did not increase the efficacy of either compound. The combination of chlorpyrifos (312 ppm) + cypermethrin (150 ppm) showed great variation between farms, with an efficacy ranging between 22-93%. Unlike chlorpyrifos, the organophosphorus ethion (622 ppm) was 99-100% effective in completely inhibiting oviposition in 4 of the 7 farms. In conclusion, resistance to deltamethrin and amitraz is very high in seven distant strains of the Colombian territory, varies widely for chlorpyrifos, and was not detected for ethion that was 99-100% effective

          Translated abstract

          O controle do carrapato Rhipicephalus (Boophilus) microplus na Colô mbia está focado quase exclusivamente no uso de acaricidas químicos. Porém, R. (B.) microplus tem desenvolvido resistência à maioria dos produtos disponíveis comercialmente. O objetivo deste estudo foi valorar o grau de resistência às concentrações recomendadas dos principais acaricidas comerciais de uso tópico na Colô mbia. Foi usada a prova da imersã o de adultas (teleoginas) recomendada pela FAO com carrapatos procedentes de 7 fazendas localizadas em 4 departamentos colombianos. A eficácia dos produtos calculou-se com base nos parâmetros reprodutivos de fecundidade e fertilidade com grupos de carrapatos (n=40) expostos a cada acaricida. Em geral, os produtos deltametrina (25 ppm) e amitraz (208 ppm) foram os menos efetivos, com valores de eficácia de  <20% e de entre 10-50%, respectivamente. Também nã o foi observado aumento da eficácia ao dobrar a concentraçã o de ambos produtos por separado. A combinaçã o de clorpirifos (312 ppm) + cipermetrina (150 ppm) mostrou uma grande variaçã o entre as fazendas, com uma eficácia de 22-93%. Diferentemente do clorpirifos, o organofosforado etion (622 ppm) teve um 99-100% de eficácia por inibiçã o completa da ovoposiçã o em 4 das 7 fazendas. Em conclusã o, a resistência à deltametrina e amitraz é muito alta em 7 populações distantes do território colombiano; quanto ao clorpirifos, varia muito e este nã o parece ter resistência cruzada com o etion que foi o de maior eficácia.<20% e de entre 10-50%, respectivamente. Também não foi observado aumento da eficácia ao dobrar a concentração de ambos produtos por separado. A combinação de clorpirifos (312 ppm) + cipermetrina (150 ppm) mostrou uma grande variação entre as fazendas, com uma eficácia de 22-93%. Diferentemente do clorpirifos, o organofosforado etion (622 ppm) teve um 99-100% de eficácia por inibição completa da ovoposição em 4 das 7 fazendas. Em conclusão, a resistência à deltametrina e amitraz é muito alta em 7 populações distantes do território colombiano; quanto ao clorpirifos, varia muito e este não parece ter resistência cruzada com o etion que foi o de maior eficácia.

          Related collections

          Most cited references21

          • Record: found
          • Abstract: not found
          • Article: not found

          Boophilus annulatus and B. microplus: laboratory tests of insecticides.

            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) guidelines for evaluating the efficacy of acaricides against ticks (Ixodidae) on ruminants.

            These guidelines have been prepared to assist in the planning, conduct and interpretation of studies for the assessment of the efficacy of acaricides (excluding vaccines and other bio-control agents) against single and multi-host ticks (Ixodidae) on ruminants. Information is provided on the selection of animals, dose determination, dose confirmation and field studies, record keeping and result interpretation. The use of pen facilities is advocated for dose determination and confirmation studies for defining therapeutic and persistent efficacy. A minimum of two studies per tick species for which claims are sought is recommended for each dose determination and dose confirmation investigation. If dose confirmation studies demonstrate greater than 95% efficacy the sponsor may proceed to field studies, where a minimum of two studies per geographical location is preferred to confirm the therapeutic and persistent efficacy under field conditions. If dose confirmation studies demonstrate less than 95% efficacy then longer-term field studies can be conducted over two tick seasons with a minimum of two studies per geographical location. These studies can incorporate other control methods such as tick vaccines, to demonstrate stable long-term tick management. Specific advice is also given on conducting studies with paralysis ticks. These guidelines are also intended to assist investigators on how to conduct specific experiments, to provide specific information for registration authorities involved in the decision-making process, to assist in the approval and registration of new acaricides, and to facilitate the worldwide adoption of standard procedures.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Acaricide resistance and synergism between permethrin and amitraz against susceptible and resistant strains of Boophilus microplus (Acari: Ixodidae).

              The control of the southern cattle tick, Boophilus microplus (Canestrini), in Mexico and many other countries relies on chemical acaricides. Boophilus microplus has developed resistance to all major classes of acaricides in recent years. To gain a better understanding of the resistance and to develop resistance management strategies that benefit both Mexican ranchers and USDA's cattle fever tick eradication program (CFTEP), the authors used larval bioassay techniques to determine levels of resistance to permethrin and amitraz and then evaluated synergism between these two acaricides in one susceptible laboratory tick strain and four resistant strains originating from Mexico and Brazil. To examine mechanisms of resistance to permethrin in these strains, the frequency of a mutated sodium channel gene was determined using a PCR assay. The tick strains from Mexico and Brazil demonstrated 49.4- to over 672.2-fold resistance to permethrin, and up to 94.5-fold resistance to amitraz. While the San Roman strain from Mexico was the most permethrin-resistant strain, the Santa Luiza strain from Brazil was the most amitraz-resistant strain. A significant correlation was found between the permethrin resistance ratio and the allelic frequency of the sodium channel mutation. Significant synergism between permethrin and amitraz was found when one acaricide was tested in the presence of another. Synergism ratios ranged from 1.5 to 54.9 when amitraz was tested as a synergist for permethrin. Similar synergism ratios were obtained when permethrin was tested as a synergist for amitraz. Permethrin caused virtually no mortality in the San Roman strain, even at the highest concentration (3294 microg cm(-2)). Adding amitraz (11.0 microg cm(-2)) to permethrin led to a dramatic increase in larval mortality, even at very low concentrations of permethrin. 2007 Society of Chemical Industry
                Bookmark

                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                cmvz
                CES Medicina Veterinaria y Zootecnia
                Ces. Med. Vet. Zootec.
                Universidad CES (Medellín, Antioquia, Colombia )
                1900-9607
                December 2016
                : 11
                : 3
                : 58-70
                Affiliations
                [02] Sede Bogotá orgnameUniversidad Nacional de Colombia Colombia
                [03] Villavicencio orgnameUniversidad de los Llanos Colombia
                [04] Montería orgnameUniversidad de Córdoba Colombia
                [01] Medellín orgnameUniversidad de Antioquia Colombia
                Article
                S1900-96072016000300007
                14d298fb-d424-48f1-a7fd-eeb0e0b74b2f

                This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

                History
                : 26 July 2016
                : 02 December 2016
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 22, Pages: 13
                Product

                SciELO Colombia


                garrapatas,bovino,cattle,acaricides,resistance,ticks,acaricidas,carrapatos,resistência,tópicos,resistencia

                Comments

                Comment on this article