Angiogenesis of Extracted Tooth Wound on Wistar Rats After Application of Okra (Abelmoschus esculentus) Gel Extract

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

Abstract Objective: To analyze angiogenesis in the post-extracted tooth of Wistar rats after application of okra (Abelmoschus esculentus) extract. Material and Methods: A total of 18 rats were divided into two groups (control and treatment). Okra extract with a concentration of 30% in gel form was applied on the post-extraction socket of the treatment group. The rats were sacrificed on day-3, day-5, and day-7 after tooth extraction. The newly-formed blood vessels were counted and statistically analyzed by means of One Way ANOVA and Tukey HSD with a significance level set at 5%. Results: The newly-formed capillaries of the control group (4.67 ± 1.53) on day-3 were lower than the treatment group (9.00 ± 1.00). The newly-formed capillaries recorded from the control group, both in day-5 (9.33 ± 1.53) and day-7 (8.67 ± 1.53) were lower than the treatment group, which started to decreased from day-5 (13.67 ± 1.53) to day-7 (12.33 ± 0.58). Significant differences were found in treatment group, on day-3 compared to day-5 (p=0.005), and on day-3 to day-7 (p=0.024). Conclusion: Okra extract in gel form at 30% concentration can increase the angiogenesis during the wound healing process of the extracted tooth on Wistar rats.

Related collections

Most cited references 22

Record: found
Abstract: found
Article: not found

The role of antioxidants in the chemistry of oxidative stress: A review.

Aurelia Pisoschi, Aneta Pop (2015)

This Review Article is focused on the action of the reactive oxygenated species in inducing oxidative injury of the lipid membrane components, as well as on the ability of antioxidants (of different structures and sources, and following different mechanisms of action) in fighting against oxidative stress. Oxidative stress is defined as an excessive production of reactive oxygenated species that cannot be counteracted by the action of antioxidants, but also as a perturbation of cell redox balance. Reactive oxygenated/nitrogenated species are represented by superoxide anion radical, hydroxyl, alkoxyl and lipid peroxyl radicals, nitric oxide and peroxynitrite. Oxidative stress determines structure modifications and function modulation in nucleic acids, lipids and proteins. Oxidative degradation of lipids yields malondialdehyde and 4-hydroxynonenal, but also isoprostanes, from unsaturated fatty acids. Protein damage may occur with thiol oxidation, carbonylation, side-chain oxidation, fragmentation, unfolding and misfolding, resulting activity loss. 8-hydroxydeoxyguanosine is an index of DNA damage. The involvement of the reactive oxygenated/nitrogenated species in disease occurrence is described. The unbalance between the oxidant species and the antioxidant defense system may trigger specific factors responsible for oxidative damage in the cell: over-expression of oncogene genes, generation of mutagen compounds, promotion of atherogenic activity, senile plaque occurrence or inflammation. This leads to cancer, neurodegeneration, cardiovascular diseases, diabetes, kidney diseases. The concept of antioxidant is defined, along with a discussion of the existent classification criteria: enzymatic and non-enzymatic, preventative or repair-systems, endogenous and exogenous, primary and secondary, hydrosoluble and liposoluble, natural or synthetic. Primary antioxidants are mainly chain breakers, able to scavenge radical species by hydrogen donation. Secondary antioxidants are singlet oxygen quenchers, peroxide decomposers, metal chelators, oxidative enzyme inhibitors or UV radiation absorbers. The specific mechanism of action of the most important representatives of each antioxidant class (endogenous and exogenous) in preventing or inhibiting particular factors leading to oxidative injury in the cell, is then reviewed. Mutual influences, including synergistic effects are presented and discussed. Prooxidative influences likely to occur, as for instance in the presence of transition metal ions, are also reminded.

0 comments Cited 705 times – based on 0 reviews      Review now

Record: found
Abstract: found
Article: found

Is Open Access

The Role of Macrophages in Acute and Chronic Wound Healing and Interventions to Promote Pro-wound Healing Phenotypes

Paulina Krzyszczyk, Rene Schloss, Andre Francis Palmer … (2018)

Macrophages play key roles in all phases of adult wound healing, which are inflammation, proliferation, and remodeling. As wounds heal, the local macrophage population transitions from predominantly pro-inflammatory (M1-like phenotypes) to anti-inflammatory (M2-like phenotypes). Non-healing chronic wounds, such as pressure, arterial, venous, and diabetic ulcers indefinitely remain in inflammation—the first stage of wound healing. Thus, local macrophages retain pro-inflammatory characteristics. This review discusses the physiology of monocytes and macrophages in acute wound healing and the different phenotypes described in the literature for both in vitro and in vivo models. We also discuss aberrations that occur in macrophage populations in chronic wounds, and attempts to restore macrophage function by therapeutic approaches. These include endogenous M1 attenuation, exogenous M2 supplementation and endogenous macrophage modulation/M2 promotion via mesenchymal stem cells, growth factors, biomaterials, heme oxygenase-1 (HO-1) expression, and oxygen therapy. We recognize the challenges and controversies that exist in this field, such as standardization of macrophage phenotype nomenclature, definition of their distinct roles and understanding which phenotype is optimal in order to promote healing in chronic wounds.

0 comments Cited 483 times – based on 0 reviews      Review now

Record: found
Abstract: found
Article: not found

Fibroblast Growth Factors: Biology, Function, and Application for Tissue Regeneration

Ye-Rang Yun, Jong-Eun Won, Eunyi Jeon … (2010)

Fibroblast growth factors (FGFs) that signal through FGF receptors (FGFRs) regulate a broad spectrum of biological functions, including cellular proliferation, survival, migration, and differentiation. The FGF signal pathways are the RAS/MAP kinase pathway, PI3 kinase/AKT pathway, and PLCγ pathway, among which the RAS/MAP kinase pathway is known to be predominant. Several studies have recently implicated the in vitro biological functions of FGFs for tissue regeneration. However, to obtain optimal outcomes in vivo, it is important to enhance the half-life of FGFs and their biological stability. Future applications of FGFs are expected when the biological functions of FGFs are potentiated through the appropriate use of delivery systems and scaffolds. This review will introduce the biology and cellular functions of FGFs and deal with the biomaterials based delivery systems and their current applications for the regeneration of tissues, including skin, blood vessel, muscle, adipose, tendon/ligament, cartilage, bone, tooth, and nerve tissues.

0 comments Cited 202 times – based on 0 reviews      Review now

All references

Author and article information

Journal

Journal ID (publisher-id): pboci

Title: Pesquisa Brasileira em Odontopediatria e Clínica Integrada

Abbreviated Title: Pesqui. Bras. Odontopediatria Clín. Integr.

Publisher: Associação de Apoio à Pesquisa em Saúde Bucal (João Pessoa, PB, Brazil )

ISSN (Print): 1519-0501

ISSN (Electronic): 1983-4632

Publication date (Print and electronic): 2020

Volume: 20

Electronic Location Identifier: e5087

Affiliations

[2] Surabaya orgnameUniversitas Airlangga orgdiv1Faculty of Dental Medicine Indonesia

[1] Surabaya orgnameUniversitas Airlangga orgdiv1Faculty of Dental Medicine orgdiv2Department of Oral Biology Indonesia

Article

Publisher ID: S1983-46322020000100338 Publisher ID: S1983-4632(20)02000000338

DOI: 10.1590/pboci.2020.037

SO-VID: 15018ead-c237-4d43-b0ae-ac2eb6b740f0

License:

This work is licensed under a Creative Commons Attribution 4.0 International License.

History

Date accepted : 13 January 2020

Date received : 19 August 2019

Page count

Figures: 0, Tables: 0, Equations: 0, References: 23, Pages: 0

Product

SciELO Brazil